Surface-enhanced Raman spectroscopy of genomic DNA from in vitro grown tomato (Lycopersicon esculentum Mill.) cultivars before and after plant cryopreservation

Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
Cristina M MunteanAdela Halmagyi

Abstract

In this work the surface-enhanced Raman scattering (SERS) spectra of five genomic DNAs from non-cryopreserved control tomato plants (Lycopersicon esculentum Mill. cultivars Siriana, Darsirius, Kristin, Pontica and Capriciu) respectively, have been analyzed in the wavenumber range 400-1800 cm(-1). Structural changes induced in genomic DNAs upon cryopreservation were discussed in detail for four of the above mentioned tomato cultivars. The surface-enhanced Raman vibrational modes for each of these cases, spectroscopic band assignments and structural interpretations of genomic DNAs are reported. We have found, that DNA isolated from Siriana cultivar leaf tissues suffers the weakest structural changes upon cryogenic storage of tomato shoot apices. On the contrary, genomic DNA extracted from Pontica cultivar is the most responsive system to cryopreservation process. Particularly, both C2'-endo-anti and C3'-endo-anti conformations have been detected. As a general observation, the wavenumber range 1511-1652 cm(-1), being due to dA, dG and dT residues seems to be influenced by cryopreservation process. These changes could reflect unstacking of DNA bases. However, not significant structural changes of genomic DNAs from Siriana, Darsiriu...Continue Reading

References

May 16, 2002·Biopolymers·C M MunteanG M J Segers-Nolten
Jan 31, 2004·Nucleic Acids Research·A J Ruiz-ChicaF J Ramírez
Mar 19, 2008·Journal of Colloid and Interface Science·Soumen BasuTarasankar Pal
Oct 18, 2011·Beilstein Journal of Nanotechnology·Regina TrefferVolker Deckert
Oct 13, 2014·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Snežana MiljanićIvona Matić

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Citations

Sep 19, 2018·Nanomaterials·Min JiaHongyan Zhang
May 17, 2019·Journal of Molecular Modeling·Cristina M MunteanNicoleta Toşa
Jun 13, 2017·Annual Review of Analytical Chemistry·Seon-Yeong KwakMichael S Strano
May 7, 2021·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Cristina M MunteanAna Coste

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