PMID: 9533624Apr 9, 1998Paper

Surface-exposed phenylalanines in the RNP1/RNP2 motif stabilize the cold-shock protein CspB from Bacillus subtilis

Proteins
T SchindlerFranz X Schmid

Abstract

In the cold-shock protein CspB from Bacillus subtilis three exposed Phe residues (F15, F17, and F27) are essential for its function in binding to single-stranded nucleic acids. Usually, the hydrophobic Phe side chains are buried in folded proteins. We asked here whether the exposition of the essential Phe residues could be a cause for the very low conformational stability of CspB. Urea-induced and heat-induced equilibrium unfolding transitions were measured for three mutants of CspB, where Phe 15, Phe 17, and Phe 27 were individually replaced by alanine. Unexpectedly, all three mutations strongly destabilized CspB. The aromatic side chains of Phe 15, Phe 17, and Phe 27 in the active site are thus important for both binding to nucleic acids and conformational stability. There is no compromise between function and stability in the active site. Model calculations indicate that, although they are partially exposed to solvent, all three Phe residues nevertheless lose accessible surface upon folding, and this should favor the native state. A different result is obtained with the F38A variant. Phe 38 is hyperexposed in native CspB, and its substitution by Ala is in fact stabilizing.

References

Jan 1, 1992·Protein Science : a Publication of the Protein Society·R Urfer, K Kirschner
Jun 5, 1992·Journal of Molecular Biology·E M MeieringA R Fersht
May 1, 1987·Journal of Bacteriology·P G JonesF C Neidhardt
Aug 5, 1987·Journal of Molecular Biology·S MillerC Chothia
Aug 1, 1995·Nature Structural Biology·T SchindlerF X Schmid
Sep 8, 1995·Journal of Molecular Biology·S WalterF X Schmid
Nov 1, 1994·Protein Science : a Publication of the Protein Society·G I Makhatadze, M A Marahiel
Jan 17, 1995·Proceedings of the National Academy of Sciences of the United States of America·B K ShoichetB W Matthews
Jan 1, 1995·Advances in Protein Chemistry·G I Makhatadze, P L Privalov
Dec 19, 1995·Biochemistry·T P CreamerG D Rose
Nov 1, 1996·Archives of Microbiology·P Graumann, M A Marahiel
Dec 24, 1996·Biochemistry·T Schindler, F X Schmid
May 27, 1997·Proceedings of the National Academy of Sciences of the United States of America·M JacobF X Schmid

❮ Previous
Next ❯

Citations

Nov 1, 2000·Nucleic Acids Research·A J Doherty, S W Suh
Apr 18, 2002·Proceedings of the National Academy of Sciences of the United States of America·Ana Rosa VigueraLuis Serrano
Dec 21, 2002·Protein Science : a Publication of the Protein Society·Markus Zeeb, Jochen Balbach
Sep 8, 2006·Nucleic Acids Research·Markus ZeebJochen Balbach
Jun 5, 2007·Journal of Bacteriology·Laura GiaquintoRicardo Cavicchioli
May 9, 2008·PLoS Computational Biology·Nobuhiko TokurikiDan S Tawfik
Mar 5, 2013·Annual Review of Biophysics·Zhixiu LiYaoqi Zhou
Aug 10, 2000·Systematic and Applied Microbiology·J A WoutersT Abee
Sep 24, 2004·Journal of Biotechnology·Vincent G H EijsinkGert Vriend
Jun 20, 2006·Journal of Molecular Biology·Klaas E A MaxUdo Heinemann
May 5, 1998·Folding & Design·B J HillierL M Gregoret
Dec 26, 2006·Journal of Molecular Biology·Alexey V Gribenko, George I Makhatadze
May 1, 2017·The FEBS Journal·Alonso I CarvajalJorge Babul
Aug 14, 2020·Chembiochem : a European Journal of Chemical Biology·Hannah Welte, Michael Kovermann
Jul 4, 2003·Science Progress·Michael H Weber, Mohamed A Marahiel
Feb 28, 2002·The Journal of Biological Chemistry·S Fabio FalsoneAndreas J Kungl
May 14, 1999·Current Opinion in Microbiology·S PhadtareM Inouye
May 29, 2018·ACS Chemical Biology·Christopher P WallisOliver Rackham
Jun 1, 2021·Canadian Journal of Microbiology·Haoyang LiMingtang Li
Dec 24, 2021·Protein Science : a Publication of the Protein Society·Victoria J Laye, Shiladitya DasSarma

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.