Surface functionalization of microgrooved titanium with dual growth factor-releasing nanoparticles for synergistic osteogenic differentiation of human mesenchymal stem cells

Colloids and Surfaces. B, Biointerfaces
Suk Won LeeSang Cheon Lee

Abstract

We demonstrate that dual release of bone morphogenic protein-2 (BMP-2) and insulin-like growth factor-1 (IGF-1) by catechol-functionalized adhesive polymer nanoparticles on microgrooved titanium (Ti) surface enhances in vitro osteoblastic differentiation of human mesenchymal stem cells (MSCs). The nanoparticles consisted of three distinct domains, surface Ti-adhesive catechol groups, anionic poly(L-aspartic acid) (PAsp) shells, and hydrophobic poly(L-phenylalanine) (PPhe) cores. The immobilization of the adhesive nanoparticles onto microgrooved Ti surface was verified using various surface analytical tools, such as field-emission scanning electron microscopy (Fe-SEM), X-ray photoelectron spectroscopy (XPS), contact angle measurement. The nanoparticles were immobilized both on the groove bottom surface and the ridge top surface with a similar anchoring density. A fluorescence microscope visualized that BMP-2 and IGF-1 of positive charges were efficiently loaded onto the negatively charged PAsp shells of immobilized nanoparticles. We confirmed the enhanced osteoblastic differentiation of MSCs by presenting the expression results of major osteoblast marker genes and proteins. In addition, overall significant correlations between t...Continue Reading

References

Jan 26, 2006·Stem Cells and Development·Hannjörg KochPhil G Campbell
Oct 20, 2007·Science·Haeshin LeePhillip B Messersmith
Dec 17, 2008·Journal of Controlled Release : Official Journal of the Controlled Release Society·Xiaoqin WangDavid L Kaplan
Aug 4, 2009·Cell and Tissue Research·Toshihisa Komori
Feb 16, 2010·Biomaterials·Myung-Hyun LeeJong-Ho Kang
Jun 4, 2013·Journal of Controlled Release : Official Journal of the Controlled Release Society·Hong Jae LeeSang Cheon Lee
Jul 23, 2014·Colloids and Surfaces. B, Biointerfaces·Yin LvRen-Xi Zhuo
Jul 25, 2014·Colloids and Surfaces. B, Biointerfaces·Lu ZhangLiyan Qiu

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