Surface Functionalization of Ti6Al4V via Self-assembled Monolayers for Improved Protein Adsorption and Fibroblast Adhesion

Langmuir : the ACS Journal of Surfaces and Colloids
Abshar HasanLalit M Pandey

Abstract

Although metallic biomaterials find numerous biomedical applications, their inherent low bioactivity and poor osteointegration had been a great challenge for decades. Surface modification via silanization can serve as an attractive method for improving the aforementioned properties of such substrates. However, its effect on protein adsorption/conformation and subsequent cell adhesion and spreading has rarely been investigated. This work reports the in-depth study of the effect of Ti6Al4V surface functionalization on protein adsorption and cell behavior. We prepared self-assembled monolayers (SAMs) of five different surfaces (amine, octyl, mixed [1:1 ratio of amine:octyl], hybrid, and COOH). Synthesized surfaces were characterized by Fourier transform infrared-attenuated total reflection (FTIR-ATR) spectroscopy, contact angle goniometry, profilometry, and field emission scanning electron microscopy (FESEM). Quantification of adsorbed mass of bovine serum albumin (BSA) and fibronectin (FN) was determined on different surfaces along with secondary structure analysis. The adsorbed amount of BSA was found to increase with an increase in surface hydrophobicity with the maximum adsorption on the octyl surface while the reverse trend w...Continue Reading

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Citations

Oct 28, 2018·Biotechnology Journal·Jean-Pierre AlcarazDonald K Martin
Sep 11, 2019·Biotechnology Journal·Balakrishnan AbinayaNagarajan Selvamurugan
Dec 24, 2019·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Kai LiYong Han
Jul 28, 2020·Journal of Biomolecular Structure & Dynamics·Laipubam Gayatri Sharma, Lalit M Pandey
Apr 17, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·Muhammad QadirCuie Wen
Nov 18, 2018·International Journal of Biological Macromolecules·Abhishek RoyLalit Mohan Pandey
Jun 2, 2021·International Journal of Biological Macromolecules·Xin ZhengZhengjun Li
Jul 24, 2021·Bioresource Technology·Rahul VermaLalit M Pandey
Jun 26, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Abshar HasanLalit M Pandey
Sep 10, 2018·ACS Biomaterials Science & Engineering·Abshar HasanLalit M Pandey
Jun 6, 2020·Langmuir : the ACS Journal of Surfaces and Colloids·Katsuhiko Ariga
Jan 24, 2019·Langmuir : the ACS Journal of Surfaces and Colloids·Rong AnJing Tao Wang
Jul 8, 2019·ACS Biomaterials Science & Engineering·Guohui ShouWenjian Weng

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