PMID: 9648025Jul 2, 1998Paper

Surface grafting of poly(ethylene glycol) onto poly(acrylamide-co-vinyl amine) cross-linked films under mild conditions

Journal of Biomaterials Science. Polymer Edition
Y Yamamoto, Michael V Sefton

Abstract

Poly(ethylene glycol) (PEG) was grafted onto poly(acrylamide-co-vinyl amine) (poly(AM-co-VA)) film using tresylated PEG (TPEG) at 37 degrees C in aqueous buffers (pH 7.4) with a view to surface-modifying microencapsulated mammalian cells. Poly(AM-co-VA) film was synthesized by Hofmann degradation of a cross-linked poly(acrylamide) film. Conversion to vinyl amine on the surface of the film was approximately 50%, but bulk conversion was not observed; surface specificity was thought to be the result of cleavage of aminated polymer chains at the surface due to chain scission. Reaction between primary amine and TPEG gave a graft yield of 2 mol% (based on XPS) with respect to available surface amine groups, equivalent to 54 mol% ethylene oxide based on monomer units. Physical adsorption of non-activated polymer was done under identical conditions as a control and the difference in oxygen content was significant compared to TPEG. The type of buffer agent and buffer concentration did not influence graft yields. This graft reaction, which was completed in as little as 2 h was considered to be mild enough to be used for a surface modification of microcapsules containing cells without affecting their viability. Such a surface modification...Continue Reading

References

Jun 1, 1992·Journal of Biomedical Materials Research·K BergströmJ M Harris
Jul 1, 1991·Journal of Biomedical Materials Research·N P Desai, J A Hubbell
Jan 1, 1987·Methods in Enzymology·K Nilsson, K Mosbach
Jan 1, 1984·Methods in Enzymology·K Nilsson, K Mosbach

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