Surface Immobilized Nucleic Acid-Transcription Factor Quantum Dots for Biosensing

Advanced Healthcare Materials
Mingfu ChenMark W Grinstaff

Abstract

Immobilization of biosensors on surfaces is a key step toward development of devices for real-world applications. Here the preparation, characterization, and evaluation of a surface-bound transcription factor-nucleic acid complex for analyte detection as an alternative to conventional systems employing aptamers or antibodies are described. The sensor consists of a gold surface modified with thiolated Cy5 fluorophore-labeled DNA and an allosteric transcription factor (TetR) linked to a quantum dot (QD). Upon addition of anhydrotetracycline (aTc)-the analyte-the TetR-QDs release from the surface-bound DNA, resulting in loss of the Förster resonance energy transfer signal. The sensor responds in a dose-dependent manner over the relevant range of 0-200 µm aTc with a limit of detection of 80 nm. The fabrication of the sensor and the subsequent real-time quantitative measurements establish a framework for the design of future surface-bound, affinity-based biosensors using allosteric transcription factors for molecular recognition.

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Citations

Mar 16, 2021·Emergent Materials·Sultan ŞahinLevent Trabzon
Sep 8, 2020·ACS Applied Materials & Interfaces·Mingfu ChenMark W Grinstaff

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