Surface localization of zein storage proteins in starch granules from maize endosperm. Proteolytic removal by thermolysin and in vitro cross-linking of granule-associated polypeptides

Plant Physiology
C Mu-Forster, B P Wasserman

Abstract

Starch granules from maize (Zea mays) contain a characteristic group of polypeptides that are tightly associated with the starch matrix (C. Mu-Forster, R. Huang, J.R. Powers, R.W. Harriman, M. Knight, G.W. Singletary, P.L. Keeling, B.P. Wasserman [1996] Plant Physiol 111: 821-829). Zeins comprise about 50% of the granule-associated proteins, and in this study their spatial distribution within the starch granule was determined. Proteolysis of starch granules at subgelatinization temperatures using the thermophilic protease thermolysin led to selective removal of the zeins, whereas granule-associated proteins of 32 kD or above, including the waxy protein, starch synthase I, and starch-branching enzyme IIb, remained refractory to proteolysis. Granule-associated proteins from maize are therefore composed of two distinct classes, the surface-localized zeins of 10 to 27 kD and the granule-intrinsic proteins of 32 kD or higher. The origin of surface-localized delta-zein was probed by comparing delta-zein levels of starch granules obtained from homogenized whole endosperm with granules isolated from amyloplasts. Starch granules from amyloplasts contained markedly lower levels of delta-zein relative to granules prepared from whole endos...Continue Reading

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Citations

Mar 31, 2005·Trends in Genetics : TIG·Bryan C Gibbon, Brian A Larkins
Nov 9, 2011·Critical Reviews in Food Science and Nutrition·Hyun-Seok KimMoo-Yeol Baik
Jun 6, 2009·Journal of Experimental Botany·Lorena MoellerKan Wang
Apr 11, 2015·Carbohydrate Polymers·Zhongquan SuiJames N BeMiller
Sep 10, 2003·Proceedings of the National Academy of Sciences of the United States of America·Rachel K ChikwambaKan Wang
Dec 12, 2012·Carbohydrate Polymers·Zhongquan Sui, James N BeMiller
May 22, 2013·Carbohydrate Polymers·Zhongquan SuiJames N Bemiller
Aug 21, 2019·Plant, Cell & Environment·Liangjie NiuWei Wang
Dec 8, 2020·Food Science and Biotechnology·Chae Eun LeeMalshick Shin
May 1, 2021·Vaccines·Jennifer Schwestka, Eva Stoger
Dec 26, 2001·Biomacromolecules·A M Smith

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