Surface modification of electrospun poly(methyl methacrylate) (PMMA) nanofibers for the development of in vitro respiratory epithelium model

Journal of Biomaterials Science. Polymer Edition
A R RabiatulB H I Ruszymah

Abstract

Scaffold design is an important aspect of in vitro model development. In this study, nanoscaffold surface modification, namely UV radiation and genipin cross-linking to immobilize collagen on the surface of electrospun poly (methyl methacrylate) (PMMA) nanofiber sheet was investigated. Samples were divided into four groups; PMMA nanofibers (PMMA), collagen-coated PMMA nanofibers (PMMACOL), genipin cross-linked collagen-coated PMMA nanofibers (PMMAGEN), and UV-irradiated collagen-coated PMMA nanofibers (PMMAUV). 6 h of UV radiation significantly reduced the hydrophobicity of PMMA nanofibers from (131.88° ± 1.33°) to (110.04° ± 0.27°) (p < 0.05). The amount of collagen immobilized was significantly higher in PMMAGEN group (239.36 ± 16.63 μg collagen/mg nanofibers) (p < 0.05) compared to the other groups. RECs on all scaffold expressed epithelial cell-specific markers (CK18 and CK14), mucin-producing cell marker (MUC5Ac) and were actively proliferating, based on the positive expression of Ki67. Total number of attached cells was significantly the highest in PMMAUV group on day 9 (6.44 × 10(4) ± 2.77 × 10(4) cells/cm(2)) and it has the highest proliferation rate from day 4 to 9 (0.005 ± 0.003 h(-1)) compared to the other groups. Ev...Continue Reading

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Citations

Oct 31, 2017·International Journal of Molecular Sciences·Nor Kamalia ZahariShiplu Roy Chowdhury
Aug 1, 2019·Current Pharmaceutical Biotechnology·Mohammad F Mh Busra, Yogeswaran Lokanathan
Sep 6, 2020·Biomedical Microdevices·Ying-Hou ChenYi-You Huang
Jan 17, 2020·International Journal of Molecular Sciences·Jegadevswari SelvarajahYogeswaran Lokanathan
Jan 12, 2020·Materials Science & Engineering. C, Materials for Biological Applications·Mohan VedhanayagamKalarical Janardhanan Sreeram
Aug 18, 2021·International Journal of Biological Macromolecules·Francesca SerioCarolina Camargo de Oliveira
Aug 10, 2019·Molecular Pharmaceutics·Xiang Yi ChenMohd Cairul Iqbal Mohd Amin

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Methods Mentioned

BETA
RECs
biopsy

Software Mentioned

ImageJ

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