Surface Modification of Multiple Bioactive Peptides to Improve Endothelialization of Vascular Grafts

Macromolecular Bioscience
Ge PengYubo Fan

Abstract

Endothelialization is an effective approach to prevent thrombus formation and enhance vascular graft survival. Surface modification of biomolecules has been proved to be effective in regulating endothelial cell behaviors. In this study, several peptides including YIGSR, RGD, and REDV sequences are covalently immobilized on the surface of electrospun silk fibroin scaffolds and the effects of combined application of these peptides on cell behaviors are studied. The results show that, compared with the scaffolds modified with single peptides, the scaffolds modified with dual peptides (YIGSR+RGD) could significantly enhance the proliferation of human umbilical vein endothelial cells (HUVECs). However, the combination of REDV+RGD or YIGSR+REDV does not promote the adhesion or proliferation of HUVECs. Notably, YIGSR-modified scaffolds improved HUVEC migration significantly in comparison to REDV- or RGD-modified groups. Moreover, its combination with either of these two peptides also presents excellent effect on cell migration. Thus, all the data suggest that the combined application of peptides might be a promising method to enhance the endothelialization of small-diameter vascular grafts.

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Citations

Aug 15, 2019·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·D I BraghirolliP Pranke
Jun 20, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Pengfei LiJian Shen
Mar 10, 2020·The International Journal of Artificial Organs·Justine Cordelle, Sara Mantero
Oct 30, 2019·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Xize GaoJun Wu
Apr 1, 2021·ACS Applied Materials & Interfaces·Jiaying ZhouYakai Feng
Apr 27, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Cong WangJian Ji
Aug 14, 2021·Acta Biomaterialia·Prerak Gupta, Biman B Mandal
Sep 9, 2021·Biochemical Society Transactions·Yuen Ting LamSteven G Wise

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