Surface modification of nanofibrous matrices via layer-by-layer functionalized silk assembly for mitigating the foreign body reaction

Biomaterials
Yuna QianYubo Fan

Abstract

The inherent hydrophobicity and large surface area of electrospun synthetic polymeric scaffolds often cause non-specific protein adsorption, thereby influencing macrophage functions and eventually leading to fibrosis at the tissue-scaffold interface. This work reports fabrication of silk fibroin (SF)-functionalized electrospun polycaprolactone (PCL) fibers by single-component layer-by-layer assembly and decorate the SF with heparin disaccharide (HD), resulting in the non-covalent binding of interleukin-4 (IL-4) with the capacity to modulate macrophage polarization. A modified SF derivative was obtained by diazonium coupling and then covalently bonded with HD via click chemistry to eventually bind IL-4 efficiently and maintain its bioactivity. In vitro studies showed that IL-4 surface-functionalized nanofibrous scaffolds promoted polarization to M2 macrophages in the short-term. Importantly, in a murine subcutaneous implantation model, we found that promoting transient shifts in macrophage polarization at early stage can significantly inhibit the extent of the late foreign body reactions. Furthermore, the results of a transcriptomic profiling showed that MARK, PI3K, JNK and NF-κB signaling pathways played an important role in re...Continue Reading

Citations

Oct 18, 2018·Advanced Healthcare Materials·Jinkyu LeeHeungsoo Shin
Apr 9, 2021·Advanced Healthcare Materials·Lars CrawfordBuddy Ratner
May 14, 2021·International Journal of Biological Macromolecules·Zhinan MaoSujun Wu
Jan 20, 2021·ACS Biomaterials Science & Engineering·Yuqing WangYubo Fan
Jan 19, 2021·ACS Biomaterials Science & Engineering·Yue ShiPeng-Yuan Wang
Nov 13, 2021·ACS Biomaterials Science & Engineering·Emily B LurierKara L Spiller

❮ Previous
Next ❯

Related Concepts

Related Feeds

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.