Surface Modification of Polymers for Tissue Engineering Applications: Arginine Acts as a Sticky Protein Equivalent for Viable Cell Accommodation

ACS Omega
Poulomi Sengupta, Bhagavatula L V Prasad

Abstract

Hydrophobic polymers, for their favorable mechanical properties, are a popular choice as permanent bioimplants. These materials remain absolutely bioinert for years, but throw up challenges when it comes to fast integration with healthy tissue. Addressing this, herein, we present a surface-modification technique of converting the hydrophobic surface of a polymeric film into a hydrophilic one using a layer-by-layer assembly process involving gold nanoparticles and small molecules like amino acids. These films showed much improved animal cell (murine fibroblast) adherence properties compared to commercially available tissue culture plates. Moreover, arginine-modified films exhibited a nearly equivalent cell viability compared to the films modified with the natural extracellular matrix component fibronectin. The surface hydrophilicity and roughness of our novel film were characterized by contact angle measurement and atomic force microscopy. Cell counting, fluorescence microscopy, cell viability, and collagen estimation assay were employed to demonstrate that our film favored a much improved cell adherence, and accommodation in comparison to the commercially available tissue culture plates.

References

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Mar 30, 2018·International Journal of Nanomedicine·Poulomi SenguptaBhagavatula Lv Prasad

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Citations

Jul 28, 2020·Frontiers in Bioengineering and Biotechnology·Mit Balvantray BhavsarJohn H Barker
Sep 3, 2020·ACS Omega·Poulomi SenguptaBhagavatula L V Prasad
Sep 8, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Yunxi Gao, Anthony Callanan

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Methods Mentioned

BETA
atomic force microscopy
transmission electron microscopy
AFM
surface plasmon resonance
fluorescence microscopy
Assay

Software Mentioned

Excel

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