Surface modification of silicone tubes by functional carboxyl and amine, but not peroxide groups followed by collagen immobilization improves endothelial cell stability and functionality

Biomedical Materials
Nasim Salehi-NikBehrouz Zandieh-Doulabi

Abstract

Surface modification by functional groups promotes endothelialization in biohybrid artificial lungs, but whether it affects endothelial cell stability under fluid shear stress, and the release of anti-thrombotic factors, e.g. nitric oxide (NO), is unknown. We aimed to test whether surface-modified silicone tubes containing different functional groups, but similar wettability, improve collagen immobilization, endothelialization, cell stability and cell-mediated NO-release. Peroxide, carboxyl, and amine-groups increased collagen immobilization (41-76%). Only amine-groups increased ultimate tensile strength (2-fold). Peroxide and amine enhanced (1.5-2.5 fold), but carboxyl-groups decreased (2.9-fold) endothelial cell number after 6 d. After collagen immobilization, cell numbers were enhanced by all group-modifications (2.8-3.8 fold). Cells were stable under 1 h-fluid shear stress on amine, but not carboxyl or peroxide-group-modified silicone (>50% cell detachment), while cells were also stable on carboxyl-group-modified silicone with immobilized collagen. NO-release was increased by peroxide and amine (1.1-1.7 fold), but decreased by carboxyl-group-modification (9.8-fold), while it increased by all group-modifications after collag...Continue Reading

References

Apr 22, 2003·In Vitro Cellular & Developmental Biology. Animal·Hua AiSteven A Jones
Nov 5, 2003·Journal of Artificial Organs : the Official Journal of the Japanese Society for Artificial Organs·Mutsumi TakagiToshiomi Yoshida
May 6, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Rommel G BacabacJenneke Klein-Nulend
May 18, 2006·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Margriet G MullenderJenneke Klein-Nulend
Jul 27, 2006·Expert Review of Medical Devices·Brittany A ZwischenbergerJoseph B Zwischenberger
Feb 24, 2007·Biomaterials·Alison P McGuigan, Michael V Sefton
Apr 17, 2007·Biomaterials·Joel L KaarWilliam J Federspiel
Jun 2, 2007·Journal of Biomaterials Science. Polymer Edition·Kwideok ParkDong Keun Han
Sep 7, 2007·Biochimica Et Biophysica Acta·R TzonevaT Groth
Apr 9, 2008·Current Topics in Medicinal Chemistry·Paul ThevenotLiping Tang
Jun 30, 2009·Respiratory Care·Peter Betit
Dec 2, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Hai LiHua Zhang
Jul 31, 2010·Science·Ralph D Lorenz
Feb 1, 2011·Advanced Drug Delivery Reviews·C V C BoutenF P T Baaijens
Feb 4, 2011·Organogenesis·Heather NolanJoseph B Zwischenberger
Oct 27, 2011·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·J J Song, H C Ott
Dec 14, 2011·Journal of Biomedical Materials Research. Part a·Mohammad FarhadiParin Yazdanifard
Feb 27, 2013·ASAIO Journal : a Peer-reviewed Journal of the American Society for Artificial Internal Organs·Ali KhachabKhosrow Mottaghy
May 20, 2014·Journal of Biomedical Materials Research. Part a·Fatemeh KabirianBehrouz Zandieh-Doulabi
Dec 11, 2014·Materials Science & Engineering. C, Materials for Biological Applications·Atefeh SoloukAlexander M Seifalian

❮ Previous
Next ❯

Citations

Jun 27, 2015·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Nasim Salehi-NikJenneke Klein-Nulend
May 25, 2016·Nanoscale Research Letters·Shuzhen ChenZhongning Lin
Jan 15, 2016·Annals of Biomedical Engineering·Nasim Salehi-NikJenneke Klein-Nulend
Oct 21, 2015·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Jing YangWencheng Zhang
Jun 11, 2020·Scientific Reports·P ČernochováL Zajíčková

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Nihon rinsho. Japanese journal of clinical medicine
Toshio Hayashi
Surgical Forum
S A MENDOZA, R A MILCH
Bulletin des sociétés d'ophtalmologie de France
P FrançoisA Blervacque
Journal of Biomedical Materials Research. Part a
Fatemeh KabirianBehrouz Zandieh-Doulabi
© 2022 Meta ULC. All rights reserved