Surface-modified complex SU-8 microstructures for indirect optical manipulation of single cells

Biomedical Optics Express
Badri L AekboteLóránd Kelemen

Abstract

We introduce a method that combines two-photon polymerization (TPP) and surface functionalization to enable the indirect optical manipulation of live cells. TPP-made 3D microstructures were coated specifically with a multilayer of the protein streptavidin and non-specifically with IgG antibody using polyethylene glycol diamine as a linker molecule. Protein density on their surfaces was quantified for various coating methods. The streptavidin-coated structures were shown to attach to biotinated cells reproducibly. We performed basic indirect optical micromanipulation tasks with attached structure-cell couples using complex structures and a multi-focus optical trap. The use of such extended manipulators for indirect optical trapping ensures to keep a safe distance between the trapping beams and the sensitive cell and enables their 6 degrees of freedom actuation.

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Citations

Mar 13, 2019·Macromolecular Rapid Communications·Dong YangYan Li
Dec 22, 2019·Micromachines·Hamish PennyEbubekir Avci
Feb 20, 2020·Micromachines·Philippa-Kate AndrewEbubekir Avci
Aug 5, 2017·Biophysics Reviews·José L Carrascosa, Mark C Leake
Oct 11, 2019·Micromachines·Edison GerenaSinan Haliyo
Mar 7, 2020·Biomedical Optics Express·Gaszton VizsnyiczaiLóránd Kelemen
Sep 26, 2020·Micromachines·István GrexaLóránd Kelemen
Mar 29, 2021·Biosensors & Bioelectronics·Qing GaoBaojun Li
May 1, 2021·Micromachines·Pooria Golvari, Stephen M Kuebler
Jun 10, 2020·ACS Applied Materials & Interfaces·Siham TelitelOlivier Soppera

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