Surface-modified yeast cells: A novel eukaryotic carrier for oral application

Journal of Controlled Release : Official Journal of the Controlled Release Society
Elisabeth E KenngottFrank Breinig

Abstract

The effective targeting and subsequent binding of particulate carriers to M cells in Peyer's patches of the gut is a prerequisite for the development of oral delivery systems. We have established a novel carrier system based on cell surface expression of the β1-integrin binding domain of invasins derived from Yersinia enterocolitica and Yersinia pseudotuberculosis on the yeast Saccharomyces cerevisiae. All invasin derivatives were shown to be effectively expressed on the cell surface and recombinant yeast cells showed improved binding to both human HEp-2 cells and M-like cells in vitro. Among the different derivatives tested, the integrin-binding domain of Y. enterocolitica invasin proved to be the most effective and was able to target Peyer's patches in vivo. In conclusion, cell surface-modified yeasts might provide a novel bioadhesive, eukaryotic carrier system for efficient and targeted delivery of either antigens or drugs via the oral route.

References

Jul 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·J C Pepe, V L Miller
Jun 1, 1997·Nature Biotechnology·E T Boder, K D Wittrup
Feb 5, 1998·Histochemistry and Cell Biology·A Gebert
Oct 9, 1999·Science·Z A HamburgerP J Bjorkman
Sep 29, 2001·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·D J Brayden
Oct 3, 2001·Microbes and Infection·D J Brayden, A W Baird
Oct 3, 2003·FEMS Immunology and Medical Microbiology·Frank BreinigManfred J Schmitt
Dec 4, 2003·FEMS Immunology and Medical Microbiology·Tanja HeintelAndreas Meyerhans
Feb 19, 2004·Advanced Drug Delivery Reviews·Mark A JepsonBarry H Hirst
Feb 24, 2006·Nature Reviews. Immunology·Marian R Neutra, Pamela A Kozlowski
Apr 4, 2006·Molecular Therapy : the Journal of the American Society of Gene Therapy·Rebecca J Critchley-ThorneGeorges Vassaux
Dec 3, 2008·Microbiology·Isabel Martinez-Argudo, Mark A Jepson
Apr 1, 2010·Future Microbiology·Isabel Martinez-Argudo, Mark A Jepson
Aug 17, 2010·FEMS Yeast Research·Andressa ArdianiJames W Hodge
Oct 20, 2010·Expert Review of Anti-infective Therapy·Cristina CunhaAgostinho Carvalho
Nov 10, 2011·Developmental and Comparative Immunology·Robert PattersonDirk Werling
Mar 3, 2012·Expert Review of Vaccines·Yoshiko FujkuyamaKohtaro Fujihashi
Jan 9, 2013·Methods in Molecular Biology·Frank BreinigManfred J Schmitt
Aug 16, 2013·Clinical and Vaccine Immunology : CVI·Haibin HuangStuart M Levitz

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Related Papers

Proceedings of the Royal Society of Medicine
J S Cornes
Nihon saikingaku zasshi. Japanese journal of bacteriology
T SaiH Ogawa
Contributions to Microbiology and Immunology
D M Ferber, R R Brubaker
BMC Research Notes
Janja TrcekKonrad Trülzsch
© 2022 Meta ULC. All rights reserved