Surface-patterned SU-8 cantilever arrays for preliminary screening of cardiac toxicity

Biosensors & Bioelectronics
Jong Yun KimDong-Weon Lee

Abstract

Arrays of a μgrooved SU-8 cantilever were utilized to analyze changes in the contraction force and beating frequency of cardiomyocytes in vitro. The longitudinally patterned μgrooves facilitates alignment of cardiomyocytes on top of the SU-8 cantilever, which increases the contraction force of cardiomyocytes by a factor of about 2.5. The bending displacement of the SU-8 cantilever was precisely measured in nanoscale using a laser-based measurement system combined with a motorized xyz stage. The cantilever displacement due to contraction of the cardiomyocytes showed the maximum on day 8 after their cultivation. Following preliminary experiments, Isoproterenol, Verapamil, and Astemizole were used to investigate the effect of drug toxicity on the physiology of cardiomyocytes. The experimental results indicated that 1 µM of Isoproterenol treatment increased contraction force and beating frequencies of cardiomyocytes by 30% and 200%, respectively, whereas 500 nM of Verapamil treatment decreased contraction force and beating frequencies of cardiomyocytes by 56% and 42%, respectively. A concentration of less than 5 nM of the hERG channel suppression drug Astemizole did not change the contraction forces in the displacement but slightly...Continue Reading

References

Jan 27, 2005·Lab on a Chip·Emilia Entcheva, Harold Bien
Sep 8, 2007·Science·Adam W FeinbergKevin Kit Parker
Jul 23, 2008·Journal of Biomechanics·Jinseok KimSukho Park
Dec 26, 2009·Stem Cell Research·Stefan R BraamChristine L Mummery
Jun 29, 2013·Lab on a Chip·Ashutosh AgarwalKevin Kit Parker

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Citations

Aug 16, 2016·Sensors·Nomin-Erdene OyunbaatarDong-Weon Lee

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