Surface plasmon enhanced diffraction for label-free biosensing

Analytical Chemistry
Fang YuWolfgang Knoll

Abstract

Surface plasmon enhanced evanescent field at a (noble) metal/dielectric interface can be employed to enhance the diffraction efficiency of surface grating structure composed of biomolecules. Based on a Kretschmann configuration, we realized a diffraction biosensor to monitor the dynamic interaction of biological molecules in a label-free way. It was demonstrated by the binding of an anti-biotin antibody to the biotin-functionalized region of a periodically patterned surface, which generated significant optical contrast to diffract the surface plasmon field. With the aid of the synchronic surface plasmon resonance signal, a quadratic dependence of diffraction signal on the amount of bound antibody was found, which coincides with the theoretical expectation. Time-dependent measurements were conducted to estimate the density of biotin thiols on the functional region.

References

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Citations

Sep 24, 2008·The Analyst·Joseph R LakowiczKazimierz Nowaczyk
May 25, 2004·Nucleic Acids Research·Fang YuWolfgang Knoll
Sep 1, 2008·Biointerphases·Iuliana E Sendroiu, Robert M Corn
Apr 19, 2011·Mass Spectrometry Reviews·Priscille GironJean-Charles Sanchez
Mar 5, 2014·Chemistry : a European Journal·Gabriel Loget, Robert M Corn
Jun 30, 2019·Optics Express·Xin TuChaolong Song
Apr 11, 2008·Analytical Chemistry·Bipin K Singh, Andrew C Hillier
Feb 26, 2009·Analytical Chemistry·Nan Zhang, Wolfgang Knoll
Jun 7, 2011·The Journal of Physical Chemistry. C, Nanomaterials and Interfaces·Yan Jun LiuTony Jun Huang
Jul 30, 2010·ACS Applied Materials & Interfaces·Teng QiuPaul K Chu

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