Surface plasmon enhanced energy transfer between gold nanorods and fluorophores: application to endocytosis study and RNA detection

Faraday Discussions
Yinan ZhangYu Chen

Abstract

Previously we have demonstrated surface plasmon enhanced energy transfer between fluorophores and gold nanorods under two-photon excitation using fluorescence lifetime imaging microscopy (FLIM) in both solution and intracellular phases. These studies demonstrated that gold nanoparticle-dye energy transfer combinations are appealing, not only in Förster resonance energy transfer (FRET) imaging, but also energy transfer-based fluorescence lifetime sensing of bio-analytes. Here, we apply this approach to study the internalization of gold nanorods (GNRs) in HeLa cells using the early endosome labeling marker GFP. The observed energy transfer between GFP and the GNRs indicates the involvement of endocytosis in GNR uptake. Moreover, a novel nanoprobe based on oligonucleotide functionalized gold nanorods for nucleic acid sensing via dye-GNRs energy transfer is demonstrated, potentially opening up new possibilities in cancer diagnosis and prognosis. The influence of oligonucleotide design on such nanoprobe performance was studied for the first time using time-resolved fluorescence spectroscopy, bringing new insights to the optimization of the nanoprobe.

References

Feb 3, 1998·Nature Biotechnology·S TyagiF R Kramer
Mar 1, 1996·Nature Biotechnology·S Tyagi, F R Kramer
Sep 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·D L SokolA M Gewirtz
Mar 30, 2000·Journal of Photochemistry and Photobiology. B, Biology·O J RolinskiJ C Pickup
Apr 3, 2001·Nature Biotechnology·B DubertretA J Libchaber
Sep 29, 2001·Advanced Drug Delivery Reviews·A T Florence, N Hussain
Mar 19, 2002·International Journal of Pharmaceutics·Jasmine Davda, Vinod Labhasetwar
Mar 7, 2003·Nature·Sean D Conner, Sandra L Schmid
Mar 3, 2005·Journal of the American Chemical Society·C S YunG F Strouse
Jun 10, 2005·Nano Letters·Richard A FarrerJohn T Fourkas
Oct 22, 2005·Proceedings of the National Academy of Sciences of the United States of America·Haifeng WangJi-Xin Cheng
Feb 9, 2006·Journal of the American Chemical Society·Xiaohua HuangMostafa A El-Sayed
May 20, 2006·Science·Nathaniel L RosiChad A Mirkin
Jul 21, 2006·The Journal of Physical Chemistry. B·Kohei ImuraHiromi Okamoto
Jul 21, 2006·The Journal of Physical Chemistry. B·Catherine J MurphyTan Li
Dec 15, 2006·Analytical Chemistry·Sarah J HurstChad A Mirkin
Nov 24, 2007·Journal of the American Chemical Society·Dwight S SeferosChad A Mirkin
Jul 4, 2008·Annals of the New York Academy of Sciences·Yu ChenRichard E Palmer
Aug 23, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Andy Wijaya, Kimberly Hamad-Schifferli
Feb 12, 2009·ACS Nano·Andy WijayaKimberly Hamad-Schifferli
Jun 16, 2009·Nature Materials·Andre E NelMike Thompson
Jul 30, 2009·Biomacromolecules·Volker Mailänder, Katharina Landfester
Aug 21, 2009·European Biophysics Journal : EBJ·A J W G VisserJ W Borst
Aug 26, 2009·ACS Nano·Andrew E PrigodichChad A Mirkin
Oct 22, 2009·Small·Ayush Verma, Francesco Stellacci
May 13, 2010·Journal of Biomedical Optics·Yinan ZhangYu Chen
Nov 3, 2010·Nanomedicine·Stefaan J H Soenen, Marcel De Cuyper
May 3, 2011·Trends in Biotechnology·Divya SivaramanWilfred Chen
Nov 8, 2011·Current Opinion in Chemical Biology·Bruce A Armitage
Jul 7, 2012·Nanoscale·Y ChenA S Barnard
Dec 28, 2009·Advanced Materials·Xiaohua HuangMostafa A El-Sayed
Nov 15, 2013·Methods and Applications in Fluorescence·Chris RacknorYu Chen
Apr 10, 2014·Methods and Applications in Fluorescence·Peng GuYu Chen

❮ Previous
Next ❯

Citations

Aug 3, 2016·Physical Chemistry Chemical Physics : PCCP·B A RussellY Chen
Apr 16, 2018·Journal of Applied Toxicology : JAT·Srinath PatibandlaXinhua Shu
Sep 9, 2016·Journal of Biomedical Optics·Guoke WeiYu Chen
May 11, 2019·Nanomedicine·Anca OnaciuIoana Berindan-Neagoe
Feb 1, 2020·Journal of Extracellular Vesicles·Maria S PanagopoulouChristopher D Gregory
May 11, 2021·Frontiers in Pharmacology·Shengnan LiaoJinfeng Liao
Aug 17, 2021·Methods and Applications in Fluorescence·Hazel L Stewart, David J S Birch

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

IEEE Engineering in Medicine and Biology Magazine : the Quarterly Magazine of the Engineering in Medicine & Biology Society
H C GerritsenC J de Grauw
© 2021 Meta ULC. All rights reserved