Surface Plasmon Resonance and Bending Loss-Based U-Shaped Plastic Optical Fiber Biosensors

Sensors
Ariadny da S ArcasMarcelo M Werneck

Abstract

Escherichia coli (E. coli) is a large and diverse bacteria group that inhabits the intestinal tract of many mammals. MostE. colistrains are harmless, however some of them are pathogenic, meaning they can make one sick if ingested. By being in the feces of animals and humans, its presence in water and food is used as indicator of fecal contamination. The main method for this microorganism detection is the bacterial culture medium that is time-consuming and requires a laboratory with specialized personnel. Other sophisticated methods are still not fast enough because they require sending samples to a laboratory and with a high cost of analysis. In this paper, a gold-coated U-shaped plastic optical fiber (POF) biosensor forE. colibacteria detection is presented. The biosensor works by intensity modulation principle excited by monochromatic light where the power absorption is imposed by predominant effect of either bending loss or surface plasmon resonance (SPR), depending on the gold thickness. Bacterial selectivity is obtained by antibody immobilization on the fiber surface. The biosensor showed a detection limit of 1.5 × 10³ colony-forming units (CFU)/mL, demonstrating that the technology can be a portable, fast response and low...Continue Reading

References

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Citations

Feb 20, 2020·Sensors·Jin-Ha ChoiJeong-Woo Choi
Mar 24, 2018·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Ying ChenHongchun Li
Oct 4, 2020·Biosensors & Bioelectronics·Jieyuan TangZhe Chen
Mar 19, 2021·ACS Infectious Diseases·Carly DeusenberyAnita Shukla
Jul 3, 2021·Biosensors·Nerea De AchaIgnacio R Matías
Jul 31, 2021·Critical Reviews in Food Science and Nutrition·Nevetha RavindranSunil C K

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Methods Mentioned

BETA
biosensor
biosensors
surface plasmon resonance

Software Mentioned

ImageJ
Arduino

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