Surface plasmon resonance and biosensor technology for real-time molecular diagnosis of beta o 39 thalassemia mutation

Molecular Diagnosis : a Journal Devoted to the Understanding of Human Disease Through the Clinical Application of Molecular Biology
G FeriottoR Gambari

Abstract

Biospecific interaction analysis (BIA) employing surface plasmon resonance (SPR) and biosensor technologies is of interest in clinical genetics. However, few data are available on its use in hereditary diseases caused by genetic mutations. The primary aim of this study was the refinement of BIA technology for use in identifying the beta o 39 mutation of the beta-globin gene, a mutation which causes a common type of beta o thalassemia. Target-biotinylated PCR products were immobilized on streptavidin-coated sensor chips and diagnosed using SPR-based BIA performed by injecting specific oligonucleotide probes into the sensor chip. We demonstrated that the beta o 39 mutation can be easily and reproducibly identified during the association phase. This should be considered a pilot study demonstrating the ability of SPR-based BIA to detect point mutations in the beta-globin gene by real-time monitoring of hybridization between oligonucleotide probes and target-biotinylated PCR products generated from genomic DNA from normal, heterozygous individuals and homozygous beta o thalassemia patients.

References

Jul 15, 1987·European Journal of Biochemistry·M Leblond-FrancillardF Rougeon
Jan 1, 1995·Analytical Biochemistry·P NilssonP A Nygren
Apr 1, 1994·PCR Methods and Applications·F J Jenkins
Apr 7, 1994·Nature·P WittungB Nordén
Jan 1, 1996·Bioorganic & Medicinal Chemistry·B Hyrup, P E Nielsen
Jan 1, 1997·Journal of Molecular Recognition : JMR·P NilssonP A Nygren
Oct 13, 2001·Laboratory Investigation; a Journal of Technical Methods and Pathology·G FeriottoR Gambari
Feb 21, 2002·Journal of Agricultural and Food Chemistry·Giordana FeriottoRoberto Gambari

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Citations

Mar 16, 2012·Analytical and Bioanalytical Chemistry·Hamdi JodaCiara K O'Sullivan
Aug 1, 2012·International Journal of Hematology·Wakako SuzukiIkuo Honma
Apr 13, 2011·Journal of Cell Communication and Signaling·Stefania MazzitelliClaudio Nastruzzi
Dec 24, 2008·Analytical and Bioanalytical Chemistry·L G CarrascosaL M Lechuga
Apr 20, 2004·Laboratory Investigation; a Journal of Technical Methods and Pathology·Giordana FeriottoRoberto Gambari

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