Surface Plasmon Resonance Imaging-MALDI-TOF Imaging Mass Spectrometry of Thin Tissue Sections

Analytical Chemistry
Simon ForestJean-Francois Masson

Abstract

Identification and quantification of proteins in imaging of biological samples are a challenge in today's science. Here, we demonstrate a novel surface plasmon resonance imaging-matrix assisted laser desorption ionization imaging mass spectrometry (SPRi-MALDI IMS) coupled technique competent for the acquisition of multiparametric information by creating a tissue section imprint on an SPRi sensor surface. Correlated images were acquired in SPRi and in MALDI IMS for abundant proteins from a single mouse kidney tissue. The spatial organization of the transferred proteins from the tissue to the SPRi surface was preserved and imaged by SPR and MALDI MS. Surface chemistry was selected to nonspecifically adsorb and retain high concentrations of proteins on the SPRi surface. The diffusion kinetics were controlled to ensure fast transfer of proteins from the tissue sections with minimal lateral diffusion to achieve high spatial fidelity transfer. Lastly, the SPRi instrument was modified to insert a tissue sample in the fluidics chamber to facilitate the real-time measurement of the transfer process. The MALDI IMS experimental conditions, such as matrix deposition and the interface between the SPRi prism and the MALDI IMS instrument, wer...Continue Reading

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Citations

Nov 2, 2016·Analytical Chemistry·Jakub PřichystalVladimír Havlíček
Nov 3, 2020·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Kazuaki Ohara, Kentaro Yamaguchi
Dec 24, 2021·Nanoscale·Qi WangKe-Ke Zhang

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