Surface plasmon resonance (SPR) as a rapid tool for serotyping of Salmonella

Biosensors & Bioelectronics
Saikat Datta MazumdarMichael Keusgen

Abstract

An SPR-based sandwich immunoassay for serotyping of Salmonella is demonstrated. The Salmonella are captured on an SPR chip using polyclonal capture antibody. SPR sensorgrams are obtained for the immunoreactions of the somatic (O) and flagellar (H) surface antigens, of the captured bacteria, to their respective antibodies. The sensorgram data are compiled to determine the antigenic formula in accordance with the Kauffmann-White scheme. Salmonella Enteritidis was completely serotyped using this SPR-based method. In addition, Salmonella belonging to serogroups B, C and D were successfully assigned to their respective serogroups. Before serotyping the bacteria are grown to a concentration of 1x10(10) mL(-1). This SPR-based serotyping provides quantitative data, and thus, eliminates the possibility of false detections as encountered in the conventional slide agglutination test (SAT). This method was also proved to work with rough strains.

Citations

Nov 26, 2010·Analytical and Bioanalytical Chemistry·Veronika LangerMichael Seidel
Oct 28, 2011·Environmental Science & Technology·Gregory MarusovMichael A Lynes
Sep 6, 2013·Physical Chemistry Chemical Physics : PCCP·Yuan ZhaoYalin Lu
Feb 26, 2014·Analytical and Bioanalytical Chemistry·Stefano Mariani, Maria Minunni
Jan 18, 2017·Veterinary World·Pravas Ranjan SahooSmruti Ranjan Mishra
Jul 2, 2020·Diagnostics·Dina M Alhaj-QasemFatin Hamimi Mustafa
Nov 2, 2018·Scientific Reports·Andrea FulgioneRosanna Capparelli

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