Surface Probe Linker with Tandem Anti-Fouling Properties for Application in Biosensor Technology

Biosensors
Sandro SpagnoloMichael Thompson

Abstract

This paper describes the anti-fouling capability of the novel monolayer-forming surface linker 3-(3-(trichlorosilylpropyloxy) propanoyl chloride (MEG-Cl). This compound was successfully attached to quartz crystal surfaces which are employed in an electromagnetic piezoelectric acoustic sensor (EMPAS) configuration. The MEG-Cl coated surface was both employed with Ni-NTA for the binding of recombinant proteins and for the tandem property of the avoidance of fouling from serum and milk. The MEG-Cl coated surfaces were found to provide a large degree of anti-fouling on the EMPAS device, and were comparable to previously studied MEG-OH surfaces. Importantly, the monolayer continued to provide anti-fouling capability to the biosensor following extension with Ni-NTA in place. Accordingly, this surface linker provides an attractive system for use in biosensor technology in terms of both its anti-fouling and linking properties.

References

Dec 20, 2011·Chemical Communications : Chem Comm·Sonia SheikhMichael Thompson
Jul 10, 2012·Chemical Society Reviews·Christophe BlaszykowskiMichael Thompson
Jan 30, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Natalia M PawlowskaMichael Thompson
Oct 15, 2014·Journal of Colloid and Interface Science·Sonia SheikhMichael Thompson
Feb 18, 2016·Biomacromolecules·Andres de los Santos PereiraCesar Rodriguez-Emmenegger
Jun 22, 2016·Biomaterials Research·Vinod B Damodaran, N Sanjeeva Murthy
Feb 23, 2020·Biosensors·Brian De La Franier, Michael Thompson
Mar 7, 2020·Journal of Clinical Neurophysiology : Official Publication of the American Electroencephalographic Society·Lilia C De la Maza KrzeptowskyDavid J Anschel

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