Surface properties of dioleoyl-sn-glycerol-3-ethylphosphocholine, a cationic phosphatidylcholine transfection agent, alone and in combination with lipids or DNA

Langmuir : the ACS Journal of Surfaces and Colloids
Robert C MacDonaldH L Brockman

Abstract

Long-chain cationic amphipaths are routinely used for transfecting DNA into cells, although the mechanism of DNA delivery by these agents is poorly understood. Since their interfacial properties are undoubtedly involved at some stage in the process, a comprehensive study of the surface behavior of at least one of these compounds is highly desirable. Hence, the behavior of the cationic transfection agent EDOPC (dioleoyl-sn-glycerol-3-ethylphosphocholine or O-ethyldioleoylphosphatidylcholine), has been characterized at the air-water interface, by itself and in mixtures with other phospholipids. Surface pressure-molecular area isotherms obtained at the argon-buffer interface revealed that EDOPC is considerably (5-10 A(2)) more expanded than the parent phosphatidylcholine (DOPC) and even more expanded than the corresponding phosphatidylglycerol (DOPG), which has a similar charge density (of opposite polarity) as EDOPC. A 1:1 mixture of EDOPC and DOPG is very slightly condensed relative to DOPG and considerably condensed relative to EDOPC. The surface/dipole potential of this mixture is the mean of those of EDOPC and DOPG and is almost the same as that of DOPC. When the composition of EDOPC mixtures was varied, several surface param...Continue Reading

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Citations

Jul 31, 2009·Chemical Communications : Chem Comm·Santanu Bhattacharya, Avinash Bajaj
Mar 29, 2014·Soft Matter·Germán Luque-CaballeroJulia Maldonado-Valderrama
Oct 4, 2011·Journal of Colloid and Interface Science·Bernard Juskowiak, Jan Paczesny
Jan 9, 2013·Chemistry : a European Journal·Philippe PierratLuc Lebeau
Mar 1, 2012·Chemistry : a European Journal·Philippe PierratLuc Lebeau
Dec 13, 2006·Biochimica Et Biophysica Acta·Rumiana KoynovaRobert C MacDonald
Feb 6, 2009·The Journal of Physical Chemistry. B·Silvia Pérez-LópezConcepció Mestres
Jan 29, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·J ZhangJ Penfold
Oct 12, 2007·Molecular Pharmaceutics·Edwin V Pozharski, Robert C MacDonald

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