PMID: 6169662Mar 15, 1981Paper

Surface proteins of simian-virus-40-transformed cells

International Journal of Cancer. Journal International Du Cancer
K ChandrasekaranP T Mora

Abstract

Mammalian cells transformed in tissue culture by SV40 were shown to contain, in addition to the SV40-coded 94,000 d large T antigen and the 20,000 d small t antigen, a approximately 56,000 d cellular protein, which specifically precipitates with sera of animals bearing SV40-induced tumor(s) (tumor or T serum). We investigated the presence of these three proteins at the surface of logarithmically growing SV40-transformed cloned mouse cells, after metabolic labelling with [35S]-methionine for 3 h. The 56,000 d protein was found to be susceptible to digestion by trypsin under conditions which did not disrupt the cells, while no small t antigen was found to be digested. Both the 56,000 d cellular protein and the SV40 large T antigen were susceptible to lactoperoxidase-catalyzed iodination from the outside of intact cells. Trypsin treatment removed both the iodinated 56,000 d protein and the iodinated SV40 large T antigen. These experiments indicated that (a certain amount of) the 56,000 d protein and a relatively small amount of the large T antigen (which is present mainly in the nucleus) are present on the cell surface. The results confirm and extend independent experiments using subcellular fractionation techniques (Luborsky and ...Continue Reading

References

Feb 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·C PrivesE Winocour
Nov 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·R Schmidt-UllrichD F Wallach
Mar 15, 1979·Nature·D P Lane, L V Crawford
May 1, 1979·Journal of Virology·H R Soule, J S Butel
Jul 15, 1969·International Journal of Cancer. Journal International Du Cancer·S KitD R Dubbs
Nov 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·R O Hynes
Sep 1, 1974·Cell·I C Henderson, D M Livingston
Jul 1, 1974·European Journal of Biochemistry·W M Bonner, R A Laskey
Apr 15, 1980·International Journal of Cancer. Journal International Du Cancer·S W Luborsky, K Chandrasekaran
Jan 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·J A MeleroR B Carroll

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Citations

Nov 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·K ChandrasekaranP T Mora
Jan 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·H JörnvallE Appella
Jan 1, 1989·Annals of the New York Academy of Sciences·J S ButelS A Maxwell
May 15, 1983·International Journal of Cancer. Journal International Du Cancer·T M Rose, R Weil
Mar 15, 1982·International Journal of Cancer. Journal International Du Cancer·H R SouleJ S Butel

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