PMID: 13549494May 25, 1958Paper

Surface responses in cultures fibroblasts elicited by ethylenediaminetetraacetic acid

The Journal of Biophysical and Biochemical Cytology
E J DORNFELD, A OWCZARZAK

Abstract

Cultures of chick heart fibroblasts were perfused with the chelating agent ethylenediaminetetraacetic acid (EDTA). Cellular responses were observed under phase optics and recorded by time-lapse cinemicrography. In interphasic fibroblasts, EDTA induces cellular contraction followed by continuous protrusion and retraction of ectoplasmic blebs ("surface bubbling"), formation of motile vermiform processes, and production of rotatory ectoplasmic swellings. The contraction and surface bubbling closely resemble the metaphase contraction and "anaphase bubbling" normally displayed by cultured fibroblasts. In dividing cells, EDTA does not affect metaphases, but anaphase bubbling appears and persists; telophasic expansion and migration of daughter cells are prevented. Initiation of new mitoses occurs during and after exposure to EDTA. No cellular responses are induced by calcium, magnesium, or ferrous chelates of EDTA. The EDTA elects are completely reversible on removal of the chelating agent, resulting in the restoration of the normal interphasic cell form and the normal expansion and migration of mitotic products. The EDTA effects are interpreted to result from the chelation and removal of divalent cations from the cell surface. Possib...Continue Reading

References

Feb 1, 1956·Proceedings of the National Academy of Sciences of the United States of America·R A Eversole, E L Tatum

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Citations

Jan 1, 1971·Archiv für die gesamte Virusforschung·H K Adldinger, B W Calnek
Jan 1, 1970·In Vitro·L Weiss
Dec 1, 1986·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·W D Meek, W L Davis
Feb 1, 1976·The Journal of Experimental Medicine·M Rabinovitch, M J DeStefano
Jun 1, 1964·J R Microsc Soc·G G ROSE
Dec 1, 1966·J R Microsc Soc·G G Rose
Feb 1, 1962·Biological Reviews of the Cambridge Philosophical Society·A S CURTIS
Jul 1, 1968·Experimental Cell Research·W D Cohen
Oct 15, 1975·Experimental Cell Research·A N Bhisey, J J Freed
Jan 1, 1979·Journal of Supramolecular Structure·W D Meek, T T Puck
Jan 1, 1978·American Journal of Hematology·L F SkinniderW Kahn
Feb 15, 1963·Annals of the New York Academy of Sciences·G G ROSE
Nov 1, 1972·Experimental Cell Research·G Clothier, H Timourian

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