Surface signaling in transcriptional regulation of the ferric citrate transport system of Escherichia coli: mutational analysis of the alternative sigma factor FecI supports its essential role in fec transport gene transcription

Molecular & General Genetics : MGG
M OchsV Braun

Abstract

Ferric citrate induces transcription of the ferric citrate transport genes (fec) in escherichia coli by binding to the outer membrane receptor protein FecA without entering the cell. The signal elicited by ferric citrate crosses the outer membrane via TonB, ExbB, and ExbD. FecR transmits the signal across the cytoplasmic membrane and activates FecI located in the cytoplasm. FecI belongs to a subgroup of sigma factors that respond to extracytoplasmic stimuli. Chromosomal insertion and deletion mutations were generated in fecI; the resulting mutants were totally devoid of FecA production and fecB-lacZ expression. Iron starvation did not derepress fec transport gene transcription in fecI mutants. Missense point mutations were generated in the predicted helix-turn-helix motif of FecI to examine its role in transcription initiation. Replacement of glutamate by alanine (E141A) at the third position in the first helix reduced the residual activity of FecI in the absence of ferric citrate to 30% of the wild-type level, but induced fec transcription almost normally n the presence of ferric citrate. Mutant FecI(K145E) displayed 156% of the activity of wild-type FecI in the absence of ferric citrate and conferred full induction by ferric ...Continue Reading

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Citations

Jan 9, 2009·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Timothy J Brickman, Sandra K Armstrong
Dec 8, 2005·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Volkmar BraunAnnette Sauter
May 24, 2006·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Volkmar BraunAnnette Sauter
Jun 1, 2006·FEMS Microbiology Letters·Sylvie MazurierPhilippe Lemanceau
Feb 24, 2000·Journal of Periodontal Research·M A CurtisJ Aduse-Opoku
Feb 9, 2007·FEMS Microbiology Letters·Fan ChengQi Jin
Jul 12, 2008·FEMS Microbiology Letters·Maarten Fauvart, Jan Michiels
May 29, 2009·FEMS Microbiology Letters·Shin OkazakiMichael Göttfert
Jan 20, 2018·The Journal of Biological Chemistry·Grace E Kenney, Amy C Rosenzweig
Feb 26, 2005·Infection and Immunity·Natalie D KingTerry D Connell
Mar 20, 2003·Journal of Bacteriology·Sabine EnzVolkmar Braun
Jan 21, 2003·Journal of Bacteriology·Carin K Vanderpool, Sandra K Armstrong
Oct 6, 2020·Frontiers in Microbiology·Frédéric J VeyrierMarcel A Behr
Sep 18, 1997·Microbiology and Molecular Biology Reviews : MMBR·J H Crosa

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