Surface Topography Hinders Bacterial Surface Motility

ACS Applied Materials & Interfaces
Yow-Ren ChangWilliam A Ducker

Abstract

We demonstrate that the surface motility of the bacterium, Pseudomonas aeruginosa, is hindered by a crystalline hemispherical topography with wavelength in the range of 2-8 μm. The motility was determined by the analysis of time-lapse microscopy images of cells in a flowing growth medium maintained at 37 °C. The net displacement of bacteria over 5 min is much lower on surfaces containing 2-8 μm hemispheres than on flat topography, but displacement on the 1 μm hemispheres is not lower. That is, there is a threshold between 1 and 2 μm for response to the topography. Cells on the 4 μm hemispheres were more likely to travel parallel to the local crystal axis than in other directions. Cells on the 8 μm topography were less likely to travel across the crowns of the hemispheres and were also more likely to make 30°-50° turns than on flat surfaces. These results show that surface topography can act as a significant barrier to surface motility and may therefore hinder surface exploration by bacteria. Because surface exploration can be a part of the process whereby bacteria form colonies and seek nutrients, these results help to elucidate the mechanism by which surface topography hinders biofilm formation.

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Citations

Jun 19, 2018·PloS One·Katherine LagreeWilliam A Ducker
Nov 24, 2018·Journal of Biomedical Materials Research. Part a·Zhiling ZhuXun Yuan
Aug 20, 2020·Biomaterials Science·Saud KhalidHuaiyu Wang
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Jan 29, 2021·Frontiers in Microbiology·Archana D SiddamJayaleka J Amarasinghe
Oct 22, 2019·Langmuir : the ACS Journal of Surfaces and Colloids·Yunyi CaoJinju Chen
Feb 23, 2019·ACS Applied Materials & Interfaces·Rachel RosenzweigAlbert F Yee
Oct 14, 2019·ACS Biomaterials Science & Engineering·Fiorela GhiliniCarolina Diaz
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Dec 9, 2019·ACS Biomaterials Science & Engineering·Yow-Ren ChangWilliam A Ducker
Aug 27, 2019·Langmuir : the ACS Journal of Surfaces and Colloids·Alex T Kriegel, William A Ducker

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