Surface translocator protein 18 kDa (TSPO) localization on immune cells upon stimulation with LPS and in ART-treated HIV+ subjects.

Journal of Leukocyte Biology
Lance K BlevinsNorbert E Kaminski

Abstract

Translocator protein 18 kDa (TSPO) is a well-known outer mitochondrial membrane protein and it is widely used as a biomarker of neuroinflammation and brain injury. Although it is thought that TSPO plays key roles in a multitude of host cell functions, including steroid biosynthesis, apoptosis, generation of reactive oxygen species, and proliferation, some of these functions have recently been questioned. Here, we report the unexpected finding that circulating immune cells differentially express basal levels of TSPO on their cell surface, with a high percentage of monocytes and neutrophils expressing cell surface TSPO. In vitro stimulation of monocytes with LPS significantly increases the frequency of cells with surface TSPO expression in the absence of altered gene expression. Importantly, the LPS increase in TSPO cell surface expression in monocytes appears to be selective for LPS because two other distinct monocyte activators failed to increase the frequency of cells with surface TSPO. Finally, when we quantified immune cell TSPO surface expression in antiretroviral therapy-treated HIV+ donors, a chronic inflammatory disease, we found significant increases in the frequency of TSPO surface localization, which could be pharmaco...Continue Reading

References

Sep 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·C Braestrup, R F Squires
Mar 1, 1978·European Journal of Immunology·B RivnayM Shinitzky
Sep 1, 1992·Molecular and Cellular Endocrinology·B O OkeV Papadopoulos
Feb 28, 1989·European Journal of Pharmacology·J C LarcherB P Croizat
Dec 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·A G MukhinK E Krueger
Jul 26, 1988·European Journal of Pharmacology·J M OlsonA B Young
Jun 1, 1987·Experimental and Molecular Pathology·J L KelleyC J Schwartz
May 1, 1996·Infection and Immunity·R LandmannW Zimmerli
Feb 6, 1998·Proceedings of the Society for Experimental Biology and Medicine·V Papadopoulos
Aug 26, 1998·Biochimica Et Biophysica Acta·G Lenaz
May 20, 1999·Toxicological Sciences : an Official Journal of the Society of Toxicology·A C Kuhlmann, T R Guilarte
Mar 21, 2000·Seminars in Immunology·P W JanesP S Kabouridis
Mar 21, 2001·Cellular Signalling·M Guha, N Mackman
Jun 20, 2001·Nature Reviews. Molecular Cell Biology·K Simons, D Toomre
Jul 11, 2002·Microbes and Infection·Dennis M KlinmanMayda Gursel
Nov 13, 2003·Proceedings of the National Academy of Sciences of the United States of America·Jeanne KwikMichael Edidin
May 14, 2004·Annual Review of Biophysics and Biomolecular Structure·Kai Simons, Winchil L C Vaz
Nov 2, 2005·Biochemical and Biophysical Research Communications·Hossein ArdehaliCarolyn Machamer
Mar 24, 2006·Toxicological Sciences : an Official Journal of the Society of Toxicology·Ming-Kai Chen, Tomás R Guilarte
Apr 1, 2008·Pharmacology & Therapeutics·Ming-Kai Chen, Tomás R Guilarte
Feb 10, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Amy-Jo CasbonMary C Dinauer
Jun 3, 2009·Retrovirology·Christopher M Coleman, Li Wu
Dec 17, 2009·Methods in Molecular Biology·Maria Célia Jamur, Constance Oliver
Dec 1, 2005·BioTechniques·Claudette M St CroixSimon C Watkins
Mar 2, 2010·Future Medicinal Chemistry·Prakash NagarkattiMitzi Nagarkatti
May 23, 2012·Glia·Sriram VennetiClayton A Wiley
Jun 22, 2012·Experimental Dermatology·Markus BöhmAndrzej Slominski

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