PMID: 8963618Sep 1, 1996Paper

Surface ultrastructures of the third-stage larvae of Gnathostoma hispidum

The Korean Journal of Parasitology
W M SohnS H Lee

Abstract

A scanning electron microscopic study was performed to observe the surface ultrastructures of the third-stage larvae of Gnathostoma hispidum. The early third-stage larvae (EL3) were collected from the viscera of Chinese loaches by the artificial digestion method. The advanced third-stage larvae (ADL3) were recovered from mice experimentally infected with EL3. Both larval worms were fixed with 2.5% glutaraldehyde, dehydrated in graded alcohol, dryed in critical point dryer, and coated with gold. The specimens were observed with a SEM (DS-130C). On the head bulb of both larval stage, the mouth had a pair of lateral lips of equal size and of half moon shape. Each lip had a couple of labial papillae and a small amphid located between the two papillae. The hooklets on the head bulb had single-pointed tips and curved posteriorly. The cuticular spines of EL3 were larger and more densely distributed in the anterior area (about 1.8 micron in length) and gradually decreased in size and number posteriorly. The cuticular spines in the anterior area of ADL3 were sharp-pointed and about 4.5 micron in length, and those in the middle area were about 1.75 micron. The velvety cuticular folds and dot-like cuticular spines were distributed in the ...Continue Reading

References

Dec 1, 1993·The Korean Journal of Parasitology·W M SohnS H Lee
Sep 1, 1996·The Korean Journal of Parasitology·W M Sohn, S H Lee

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Citations

Jan 27, 2004·The Journal of Parasitology·E T HanJ Y Chai

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