PMID: 8948468Nov 13, 1996Paper

Surfactant protein B: effects on lipid domain formation and intermembrane lipid flow

Biochimica Et Biophysica Acta
L A CreuwelsH P Haagsman

Abstract

Pulmonary surfactant is a mixture of (phospho)lipids and surfactant specific proteins, lining the alveolar space. During each respiration cycle phospholipids are transferred between the phospholipid monolayer at the air/water interface and a variety of underlying membranes. Surfactant proteins may play a role in facilitating the insertion and removal of phospholipids by affecting the lipid organization of the bilayer and monolayer. The experiments described in this article were carried out in order to investigate the influence of surfactant protein B (SP-B) on the distribution of phospholipids in membranes and on the mixing of lipids between membranes. To determine the distribution of the non-labeled phospholipids in small unilamellar vesicles (SUV), the relative clustering of pyrene-labeled phospholipids was used, by measuring the ratio of excimer-to-monomer (E/M) pyrene fluorescence. In the absence of SP-B it was found that the clustering of the pyrenePC molecules was dependent on the proportion of saturated acyl chains and not on the proportion of negative charges. Addition of the positively charged SP-B to a mixture of DPPC and PG, led to an increase of approximately 20% in E/M ratio, indicating a clustering of the negative...Continue Reading

References

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Citations

Sep 13, 2002·Journal of Molecular Biology·Yiannis N KaznessisRonald G Larson
Feb 22, 2001·Annual Review of Physiology·T E Weaver, J J Conkright
Sep 16, 2003·American Journal of Respiratory Cell and Molecular Biology·Frank BraschMatthias Ochs
May 29, 2000·Biochimica Et Biophysica Acta·S ZaltashB Persson
Nov 14, 1998·Biochimica Et Biophysica Acta·J Goerke
Oct 13, 2000·Biochimica Et Biophysica Acta·E J Veldhuizen, H P Haagsman
Oct 12, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Z WangR H Notter
Mar 24, 2021·Archives of Biochemistry and Biophysics·José Carlos Castillo-SánchezJesús Pérez-Gil
Apr 8, 1999·Progress in Lipid Research·J J Batenburg, H P Haagsman

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