PMID: 9184791May 1, 1997Paper

Surfactant secretion by type II pneumocytes is inhibited by high glucose concentrations

Experimental Lung Research
I H Gewolb, J O'Brien

Abstract

Delayed fetal lung maturation is observed in poorly controlled diabetic pregnancies. To investigate whether elevated glucose levels inhibit basal surfactant secretion and synthesis in type II cells and whether inhibitory effects on secretion can be reversed by secretagogues, type II cells isolated from 20-day fetal rat lung explants were initially cultured in [H3] choline containing media with glucose concentrations of 5.5, 10, 25, 50, and 100 mM, or in equiosmolar mannitol controls. Further incubation in nonradioactive media containing matched glucose levels with and without 1 x 10(-5) M terbutaline 1 x 10(-6) M and 1 x 10(-8) M 12-O-tetradecanoylphorbol 13-acetate (TPA) allowed assessment of incorporation of choline into phosphatidylcholine (PC) and its subsequent secretion. PC secretion was inhibited by culture in high glucose conditions, resulting in an approximately 30% reduction in secretion under 50 and 100 mM glucose conditions compared to culture at 5.5 or 10 mM glucose (p < .01); this decrease could not be explained by changes in osmolarity or in all viability after culture in high glucose. Insulin (1 unit/mL) had no significant impact on secretion (92 +/- 7% of control). Terbutaline-stimulated cells grown under 50 an...Continue Reading

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Citations

Nov 19, 2011·Current Diabetes Reports·William W Hay
Nov 26, 1999·Clinical Obstetrics and Gynecology·M D BerkusO Langer
Sep 26, 2013·Journal de gynécologie, obstétrique et biologie de la reproduction·M DoreyF Pierre
Mar 3, 2009·Seminars in Fetal & Neonatal Medicine·A Michael Weindling
Apr 27, 2007·Archives de pédiatrie : organe officiel de la Sociéte française de pédiatrie·C BarondiotJ-M Hascoet
Aug 28, 2015·Journal of Perinatology : Official Journal of the California Perinatal Association·G A VilchezR J Sokol
May 24, 2018·Neonatal Network : NN·Christopher McPherson, Jennifer A Wambach
Jul 6, 2014·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Erin V McGillickSandra Orgeig
Dec 3, 2021·Radiographics : a Review Publication of the Radiological Society of North America, Inc·Hassan AboughaliaMariam Moshiri

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