Surfactin from Bacillus subtilis induces apoptosis in human oral squamous cell carcinoma through ROS-regulated mitochondrial pathway

Journal of Cancer
Thi Thuy Tien VoI-Ta Lee

Abstract

Recently, ambient air particulate matter (PM) has been shown to increase the risk of oral cancer. The most common malignant tumor in the oral cavity is oral squamous cell carcinoma (OSCC). Recent studies have revealed that surfactin, a cyclic lipopeptide generated by Bacillus subtilis, has anti-inflammatory and anti-cancer properties. However, the exact anti-cancer effects of surfactin on human OSCC and underlying molecular mechanisms remain largely unknown. In the present study, we found that treatment of SCC4 and SCC25 cells (human OSCC cell lines) with surfactin reduced the viability of SCC4 and SCC25 cells by induction of apoptosis. Surfactin-induced apoptosis was associated with caspase activation and poly(ADP-ribose) polymerase (PARP) cleavage and was regulated by the mitochondrial pathway, exemplified by mitochondrial depolarization, mitochondrial-derived reactive oxidative species (ROS) production, cytochrome c release, up-regulation of Bad and Bax, and down-regulation of Bcl-2. Surfactin induced NADPH oxidase-dependent ROS generation, which appeared essential for the activation of the mitochondrial pathway. Surfactin-induced mitochondrial-derived ROS generation was associated with JNK1/2 activation. After treatment wit...Continue Reading

Citations

Jan 30, 2021·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Hsin-Yu ChouHui-Min David Wang
Jul 25, 2021·International Journal of Molecular Sciences·Hai HuangMyoung Ok Kim

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Methods Mentioned

BETA
surgical resection
enzyme-linked immunosorbent assay
protein assay
transfection

Software Mentioned

GraphPad Prism
GraphPad

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