Surfactosomes: a novel approach to the reconstitution and 2-D crystallisation of membrane proteins

FEBS Letters
I ReviakineA Holzenburg

Abstract

The formation of vesicle-like structures (termed surfactosomes) and lamellar sheets from solutions containing ammonium perfluoroocanoate (APFO) is illustrated using conventional and cryo-transmission electron microscopy. It is shown how this detergent can be used for the solubilisation, reconstitution and 2-D crystallisation of membrane proteins as demonstrated for the major protein of the membrane sector of the V-type H+-ATPase (16-kDa protein). Electron microscopical analysis of 2-D crystals of the 16-kDa protein (a=b=13.0+/-0.2nm with gamma = 90 degrees and p4 projection symmetry) revealed a unit cell comprising four dimeric complexes of the 16-kDa protein the significance of which is discussed.

References

Jan 1, 1991·Molecular Carcinogenesis·M E FinbowJ B Findlay
Jan 1, 1983·Journal of Microscopy·J LepaultJ Dubochet
Jan 1, 1984·Methods in Enzymology·A J FurthJ D Priddle
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Nov 2, 1995·Nature·B SternbergA T Florence
Apr 1, 1993·European Journal of Biochemistry·A HolzenburgM E Finbow
Jan 1, 1995·Analytical Biochemistry·F H Shepherd, A Holzenburg

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Citations

Apr 17, 1999·International Journal of Pharmaceutics·M N Jones
Feb 13, 2001·Micron : the International Research and Review Journal for Microscopy·G Mosser
Jan 13, 2015·The Journal of Membrane Biology·Narahari AkkaladeviMark T Fisher

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