Surprising gene expression patterns within and between PDF-containing circadian neurons in Drosophila.

Proceedings of the National Academy of Sciences of the United States of America
Elzbieta Kula-EversoleMichael Rosbash

Abstract

To compare circadian gene expression within highly discrete neuronal populations, we separately purified and characterized two adjacent but distinct groups of Drosophila adult circadian neurons: the 8 small and 10 large PDF-expressing ventral lateral neurons (s-LNvs and l-LNvs, respectively). The s-LNvs are the principal circadian pacemaker cells, whereas recent evidence indicates that the l-LNvs are involved in sleep and light-mediated arousal. Although half of the l-LNv-enriched mRNA population, including core clock mRNAs, is shared between the l-LNvs and s-LNvs, the other half is l-LNv- and s-LNv-specific. The distribution of four specific mRNAs is consistent with prior characterization of the four encoded proteins, and therefore indicates successful purification of the two neuronal types. Moreover, an octopamine receptor mRNA is selectively enriched in l-LNvs, and only these neurons respond to in vitro application of octopamine. Dissection and purification of l-LNvs from flies collected at different times indicate that these neurons contain cycling clock mRNAs with higher circadian amplitudes as well as at least a 10-fold higher fraction of oscillating mRNAs than all previous analyses of head RNA. Many of these cycling l-LN...Continue Reading

References

Nov 24, 2001·Neuron·A Claridge-ChangM W Young
Feb 21, 2002·The Journal of Biological Chemistry·Hiroki R UedaSeiichi Hashimoto
Apr 23, 2002·Nature·Kai-Florian StorchCharles J Weitz
Jun 29, 2002·Proceedings of the National Academy of Sciences of the United States of America·Yiing LinPaul H Taghert
Nov 26, 2002·Nature·Jui-Ming LinRavi Allada
Feb 4, 2003·Nature Neuroscience·Bikem AktenF Rob Jackson
Mar 19, 2003·Development·Randall S HewesPaul H Taghert
Jun 18, 2003·Cell·Jie ZhaoRavi Allada
Nov 10, 2004·Journal of Biological Rhythms·Paul E Hardin
Feb 22, 2005·Genes, Brain, and Behavior·C Helfrich-Förster
Apr 11, 2006·Journal of Biological Rhythms·Jerry H HoulPaul E Hardin
May 23, 2006·Trends in Neurosciences·Sacha B NelsonChris M Hempel
Aug 30, 2006·Proceedings of the National Academy of Sciences of the United States of America·Sebastian KadenerMichael Rosbash
May 8, 2007·Neurochemical Research·Tahira Farooqui
Jan 24, 2008·Current Biology : CB·Michael N Nitabach, Paul H Taghert
Apr 19, 2008·Cold Spring Harbor Symposia on Quantitative Biology·M RosbashC-H A Tang
Sep 6, 2008·Current Biology : CB·Vasu SheebaTodd C Holmes
Sep 19, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Amanda Crocker, Amita Sehgal
Dec 9, 2008·Proceedings of the National Academy of Sciences of the United States of America·Yuhua ShangMichael Rosbash
Jan 13, 2009·General and Comparative Endocrinology·Dongkook Park, Paul H Taghert
Mar 20, 2009·PLoS Biology·Michael Rosbash
Apr 4, 2009·PLoS Genetics·Michael E HughesJohn B Hogenesch
Sep 17, 2009·Journal of Biological Rhythms·Valerie L Kilman, Ravi Allada

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Citations

Mar 4, 2011·Proceedings of the National Academy of Sciences of the United States of America·Dinara ShakiryanovaEdwin S Levitan
Jun 14, 2012·Proceedings of the National Academy of Sciences of the United States of America·Jonathon L BurmanLeo Pallanck
Jan 9, 2013·Proceedings of the National Academy of Sciences of the United States of America·Joseph RodriguezMichael Rosbash
Nov 21, 2012·Briefings in Functional Genomics·Soledad Perez-SantángeloMarcelo J Yanovsky
Nov 17, 2011·Genes & Development·Katharine Compton AbruzziMichael Rosbash
Nov 17, 2011·Genes & Development·Isaac Edery
Nov 17, 2012·Genes & Development·Weifei LuoMichael Rosbash
Apr 5, 2012·Genome Research·Michael E HughesMichael N Nitabach
Dec 18, 2013·PLoS Biology·Esteban J BeckwithMaría Fernanda Ceriani
Mar 8, 2013·PLoS Computational Biology·Ying LiJun Yan
Jun 1, 2011·PloS One·Justin R DiAngeloAmita Sehgal
Dec 12, 2012·PloS One·Laura M BeaverJadwiga M Giebultowicz
Jan 10, 2013·PloS One·Samira Chahad-EhlersRafaela Vieira Bruno
Sep 27, 2012·Journal of Biological Rhythms·Marc RubenJustin Blau
Nov 3, 2010·Progress in Neurobiology·E MeelkopT Janssen
Apr 29, 2011·EMBO Reports·Angélique LamazeFrançois Rouyer
Jun 30, 2015·Current Opinion in Insect Science·Ozgur Tataroglu, Patrick Emery
Oct 8, 2013·Neuron·Yuhua ShangLeslie C Griffith
Jun 4, 2013·Neuroscience·N I MuraroM F Ceriani
Dec 18, 2013·Current Biology : CB·Antonio C A Meireles-FilhoAlexander Stark
Sep 3, 2011·Current Biology : CB·Dongkook ParkPaul H Taghert
Oct 9, 2012·Neuron·Paul H Taghert, Michael N Nitabach
Apr 8, 2014·Proceedings of the National Academy of Sciences of the United States of America·Yue LiMichael Rosbash
Apr 24, 2016·Neuroscience Research·Ikumi MabuchiTakaomi Sakai

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