Surprising roles for phospholipid binding proteins revealed by high throughput genetics

Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire
Marissa A LeBlanc, Christopher R McMaster

Abstract

Saccharomyces cerevisiae remains an ideal organism for studying the cell biological roles of lipids in vivo, as yeast has phospholipid metabolic pathways similar to mammalian cells, is easy and economical to manipulate, and is genetically tractable. The availability of isogenic strains containing specific genetic inactivation of each non-essential gene allowed for the development of a high-throughput method, called synthetic genetic analysis (SGA), to identify and describe precise pathways or functions associated with specific genes. This review describes the use of SGA to aid in elucidating the function of two lipid-binding proteins that regulate vesicular transport, Sec14 and Kes1. Sec14 was first identified as a phosphatidylcholine (PC) - phosphatidylinositol (PI) transfer protein required for viability, with reduced Sec14 function resulting in diminished vesicular transport out of the trans-Golgi. Although Sec14 is required for cell viability, inactivating the KES1 gene that encodes for a member of the oxysterol binding protein family in cells lacking Sec14 function results in restoration of vesicular transport and cell growth. SGA analysis identified a role for Kes1 and Sec14 in regulating the level and function of Golgi P...Continue Reading

References

Apr 1, 1989·Genetics·P NovickD Botstein
Oct 1, 1993·Trends in Genetics : TIG·L Guarente
Oct 15, 1998·Proceedings of the National Academy of Sciences of the United States of America·Z XieV A Bankaitis
Dec 10, 1999·Nature Cell Biology·C Walch-Solimena, P Novick
Jan 25, 2002·The Journal of Cell Biology·Edina Harsay, Randy Schekman
Feb 16, 2002·The EMBO Journal·Sangiliyandi GurunathanJeffrey E Gerst
Feb 7, 2004·Science·Amy Hin Yan TongCharles Boone
Dec 14, 2004·Nature Genetics·Daniel SegrèRoy Kishony
Dec 22, 2004·The Journal of Biological Chemistry·J Pedro Fernández Murray, Christopher R McMaster
Jul 16, 2005·BioTechniques·Andreas Knödler, Peter Mayinger
Sep 13, 2005·Nature Genetics·Armaity P DavierwalaTimothy R Hughes
Sep 21, 2005·The Journal of Biological Chemistry·J Pedro Fernández-Murray, Christopher R McMaster
Dec 21, 2005·The Journal of Cell Biology·Thomas StrahlJeremy Thorner
Feb 3, 2006·Molecular Biology of the Cell·Micah RobinsonJeffrey E Gerst
Oct 20, 2006·Nature Chemical Biology·Kristina E IleVytas A Bankaitis
Jan 16, 2007·Nature Structural & Molecular Biology·Guillaume DrinBruno Antonny
Apr 13, 2007·Biochimica Et Biophysica Acta·Kan SaitoTomas Mustelin
May 22, 2007·Biochimica Et Biophysica Acta·Carl J MousleyVytas A Bankaitis
Jul 14, 2007·Vitamins and Hormones·D Manor, S Morley
Sep 21, 2007·Proceedings of the National Academy of Sciences of the United States of America·Gregory D FairnChristopher R McMaster

❮ Previous
Next ❯

Citations

Feb 14, 2012·Integrative Biology : Quantitative Biosciences From Nano to Macro·Jordan L ScottRobert V Stahelin
Sep 30, 2015·Chemistry and Physics of Lipids·Gerard J Gaspard, Christopher R McMaster
Jan 15, 2014·Chemistry and Physics of Lipids·Susan A HenryStephen A Jesch
Dec 5, 2012·Reproductive Health Matters·Sarah MacCarthySofia Gruskin
Feb 1, 2011·Critical Reviews in Biochemistry and Molecular Biology·Shamshad Cockcroft, Kathryn Garner

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.