Surveillance for Sri Lankan cassava mosaic virus (SLCMV) in Cambodia and Vietnam one year after its initial detection in a single plantation in 2015

PloS One
Nami MinatoStef de Haan

Abstract

Cassava mosaic disease, one of the ten most economically important crop viral diseases in the world, was first reported in Southeast Asia from a single plantation in Cambodia in 2015. To determine the presence and incidence of Sri Lankan cassava mosaic virus (SLCMV) one year after first detection, a total of 6,480 samples from 419 fields were systematically collected from cassava production areas across Cambodia (3,840 samples; 240 fields) and Vietnam (2,640samples; 179 fields) in the 2016 cropping season. Using PCR-based diagnostics, we identified 49 SLCMV-infected plants from nine fields, representing 2% of the total number of fields sampled. Infected fields were geographically restricted to two provinces of Eastern Cambodia, while no infection was detected from any of the other sampled sites in either country. Symptom expression patterns in infected plants suggested that SLCMV may have been transmitted both through infected planting materials, and by Bemisia tabaci, the known whitefly vector of SLCMV. In addition, 14% of virus infected plants did not express typical symptoms of cassava mosaic disease on their leaves, highlighting that molecular-based validation is needed to confirm the presence of SLCMV in the field. None of...Continue Reading

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Citations

Jun 12, 2020·Breeding Science·Al Imran MalikManabu Ishitani
Jul 10, 2021·Plant Molecular Biology·Ayaka UkeMasashi Ugaki
Jul 29, 2021·PeerJ·Cesar A D XavierFrancisco M Zerbini
Jul 28, 2021·Plant Molecular Biology·Vasthi Alonso ChavezC Finn McQuaid
Oct 12, 2021·PloS One·Kingkan SaokhamWanwisa Siriwan

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Datasets Mentioned

BETA
MH351658-MH351666
KT861468.1
NC001938

Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

QGIS
ClustalW
MEGA7
MEGA7 Genetics Analysis

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