Surveillance of Cancer Stem Cell Plasticity Using an Isoform-Selective Fluorescent Probe for Aldehyde Dehydrogenase 1A1

ACS Central Science
Chelsea AnormaJefferson Chan

Abstract

Cancer stem cells (CSCs) are progenitor cells that contribute to treatment-resistant phenotypes during relapse. CSCs exist in specific tissue microenvironments that cell cultures and more complex models cannot mimic. Therefore, the development of new approaches that can detect CSCs and report on specific properties (e.g., stem cell plasticity) in their native environment have profound implications for studying CSC biology. Herein, we present AlDeSense, a turn-on fluorescent probe for aldehyde dehydrogenase 1A1 (ALDH1A1) and Ctrl-AlDeSense, a matching nonresponsive reagent. Although ALDH1A1 contributes to the detoxification of reactive aldehydes, it is also associated with stemness and is highly elevated in CSCs. AlDeSense exhibits a 20-fold fluorescent enhancement when treated with ALDH1A1. Moreover, we established that AlDeSense is selective against a panel of common ALDH isoforms and exhibits exquisite chemostability against a collection of biologically relevant species. Through the application of surface marker antibody staining, tumorsphere assays, and assessment of tumorigenicity, we demonstrate that cells exhibiting high AlDeSense signal intensity have properties of CSCs. Using these probes in tandem, we have identified C...Continue Reading

References

Jan 1, 1997·Advances in Experimental Medicine and Biology·Z J LiuB C Wang
Aug 4, 1999·Proceedings of the National Academy of Sciences of the United States of America·R W StormsC Smith
Jan 13, 2001·Chemico-biological Interactions·V VasiliouD R Petersen
Dec 25, 2003·Journal of the American Chemical Society·Ellen K Feuster, Timothy E Glass
Jan 15, 2004·Journal of the American Chemical Society·Oleksandr RusinRobert M Strongin
Mar 25, 2004·Journal of the American Chemical Society·Fujie TanakaCarlos F Barbas
Oct 28, 2004·Journal of the American Chemical Society·Tasuku UenoTetsuo Nagano
Jul 12, 2005·Human Genomics·Vasilis Vasiliou, Daniel W Nebert
Nov 10, 2005·Journal of the American Chemical Society·Weihua WangRobert M Strongin
Jan 9, 2008·Trends in Pharmacological Sciences·Greg M ThurberK Dane Wittrup
Nov 6, 2008·Chemico-biological Interactions·Ming-Fang WangShih-Jiun Yin
Jul 4, 2009·Journal of the American Chemical Society·Saki IzumiTetsuo Nagano
Dec 17, 2009·Laboratory Investigation; a Journal of Technical Methods and Pathology·Ting LiFeng Jiang
Jun 24, 2010·Breast Cancer Research and Treatment·Liheng ZhouJinsong Lu
Oct 6, 2010·Proceedings of the National Academy of Sciences of the United States of America·Huiping LiuMichael F Clarke
Mar 10, 2011·Nature Medicine·Hans Clevers
Nov 15, 2011·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Thaer KhouryXinhui Wang
May 24, 2012·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·Xiao LiXiaoyan Bai
Jun 6, 2012·Journal of Mammary Gland Biology and Neoplasia·Frances L ShawRobert B Clarke
Jun 4, 2013·Blood Cells, Molecules & Diseases·Xue Yan CuiLing Sun
Jan 29, 2014·Proceedings of the National Academy of Sciences of the United States of America·Simone GaedickeGabriele Niedermann
Apr 25, 2014·Nature Communications·Il MinnMartin G Pomper
Sep 27, 2014·Drug Discovery Today·Klaus Pors, Jan S Moreb
Dec 3, 2014·Chemico-biological Interactions·Cynthia A Morgan, Thomas D Hurley
Dec 17, 2014·Chemico-biological Interactions·Cynthia A MorganThomas D Hurley
Jan 3, 2015·ACS Chemical Biology·Min LuoJohn J Tanner
Aug 21, 2015·Cancer Research·Kiera Rycaj, Dean G Tang
Sep 13, 2015·Advanced Drug Delivery Reviews·Kazuyoshi KiseNobuyuki Takakura
Sep 24, 2015·Seminars in Cancer Biology·Ira SkvortsovaSergej Skvortsov
Dec 5, 2015·BMB Reports·Juergen FinkBon-Kyoung Koo
Apr 5, 2016·Nature Materials·Junmin LeeKristopher A Kilian
Jul 16, 2016·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Amancio Carnero, Matilde Lleonart
Jul 23, 2016·The International Journal of Biochemistry & Cell Biology·Radosſaw JanuchowskiMaciej Zabel
Oct 21, 2016·Scientific Reports·Andrea MorettiSilvia Garavaglia
Mar 25, 2017·Stem Cells International·Eunice Yuen-Ting LauTerence Kin-Wah Lee
May 13, 2017·Science·Peter J ThulEmma Lundberg
Oct 28, 2017·Frontiers in Immunology·Katerina T XenakiPaul M P van Bergen En Henegouwen
Nov 9, 2017·International Journal of Molecular Sciences·Romana-Rea Begicevic, Marco Falasca

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Citations

Dec 7, 2018·Chemistry : a European Journal·Raul PereiraTimothy H Witney
Jul 8, 2020·Chemical Society Reviews·Jiyou HanJong Seung Kim
Dec 20, 2019·Angewandte Chemie·Anuj K YadavJefferson Chan
Dec 18, 2020·European Journal of Medicinal Chemistry·Bingyan LiZonghui Ma
Jan 2, 2020·ACS Central Science·Sebastiaan T A KoendersMario van der Stelt
Jan 28, 2021·Bioconjugate Chemistry·Atsushi YagishitaYasuteru Urano
Aug 10, 2021·World Journal of Stem Cells·Nohemí Salinas-JazmínMarco Velasco-Velázquez

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Methods Mentioned

BETA
Fluorescence
flow cytometry
confocal microscopy
column chromatography
Assay

Software Mentioned

ImageJ
AlDeSense
Living Image

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