Surveillance of Enterococcus spp. reveals distinct species and antimicrobial resistance diversity across a One-Health continuum.

Scientific Reports
Rahat ZaheerTim A McAllister

Abstract

For a One-Health investigation of antimicrobial resistance (AMR) in Enterococcus spp., isolates from humans and beef cattle along with abattoirs, manured fields, natural streams, and wastewater from both urban and cattle feedlot sources were collected over two years. Species identification of Enterococcus revealed distinct associations across the continuum. Of the 8430 isolates collected, Enterococcus faecium and Enterococcus faecalis were the main species in urban wastewater (90%) and clinical human isolates (99%); Enterococcus hirae predominated in cattle (92%) and feedlot catch-basins (60%), whereas natural streams harbored environmental Enterococcus spp. Whole-genome sequencing of E. faecalis (n = 366 isolates) and E. faecium (n = 342 isolates), revealed source clustering of isolates, indicative of distinct adaptation to their respective environments. Phenotypic resistance to tetracyclines and macrolides encoded by tet(M) and erm(B) respectively, was prevalent among Enterococcus spp. regardless of source. For E. faecium from cattle, resistance to β-lactams and quinolones was observed among 3% and 8% of isolates respectively, compared to 76% and 70% of human clinical isolates. Clinical vancomycin-resistant E. faecium exhibit...Continue Reading

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Citations

Jul 30, 2020·Microorganisms·Sónia RamosPatrícia Poeta
Oct 18, 2020·Antibiotics·Shashi B KumarOuliana Ziouzenkova
Dec 18, 2020·Frontiers in Veterinary Science·Sherry J HannonSheryl P Gow
Aug 25, 2021·Journal of Global Antimicrobial Resistance·Michael BiggelRoger Stephan
Aug 10, 2021·Journal of Bacteriology·Cydney N JohnsonAnushila Chatterjee

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Datasets Mentioned

BETA
CP003583.1
CP002621.1
PRJNA313047
PRJNA604849

Methods Mentioned

BETA
PCR
Illumina sequencing

Software Mentioned

Trimmomatic
PhyML
BLAST
ABRicate
PubMLST
FigTree
SNVPhyl
SPAdes

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