Surveillance of measles in the Sudan using filter paper blood samples

Journal of Medical Virology
H S El MubarakR L De Swart

Abstract

Dried blood spots collected on filter paper are considered potential clinical specimens for measles surveillance because of their ease of collection, storage, and transport. The usefulness of these samples for surveillance of measles was evaluated in a field setting. Blood spots were collected by finger-prick from 316 clinically diagnosed measles patients in suburban Khartoum, mostly within a week after onset of the rash. Samples were collected between October, 2000 and April, 2003, and stored at 4 degrees C. Measles virus-specific IgM antibodies were detected in 200 (63%) of the samples using an "in-house" IgM capture ELISA. For 201 samples reconstitution and IgM measurement was repeated 1 year after initial testing with essentially the same results, showing the stability of IgM in the filter paper under these conditions. In a limited number of samples (n = 38) measles virus-specific IgM was also tested with a commercial indirect IgM ELISA. Although the results of the two assays correlated well, the "in-house" IgM capture ELISA proved slightly more sensitive. Measles virus-specific reverse transcriptase polymerase chain reaction (RT-PCR) amplicons were obtained from 16 of 57 (28%) samples tested. Sequencing of the 3' 456 nucle...Continue Reading

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Citations

Jun 17, 2011·The Journal of Infectious Diseases·Paul A RotaDavid Featherstone
Jan 13, 2012·Clinical and Vaccine Immunology : CVI·Brian Andrew ChaseJoseph Barten Legutki
Oct 2, 2008·Malaria Journal·Patrick H CorranEleanor Riley
Jan 17, 2012·Antiviral Research·Ingrid J M SnijdewindRob A Gruters
May 26, 2012·Journal of Clinical Laboratory Analysis·Sogol SheikhakbariShohreh Shahmahmoodi
Jul 25, 2006·Journal of Virological Methods·Sara MercaderWilliam J Bellini

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