Surveillance of nuclear pore complex assembly by ESCRT-III/Vps4

Cell
Brant M WebsterC Patrick Lusk

Abstract

The maintenance of nuclear compartmentalization by the nuclear envelope and nuclear pore complexes (NPCs) is essential for cell function; loss of compartmentalization is associated with cancers, laminopathies, and aging. We uncovered a pathway that surveils NPC assembly intermediates to promote the formation of functional NPCs. Surveillance is mediated by Heh2, a member of the LEM (Lap2-emerin-MAN1) family of integral inner nuclear membrane proteins, which binds to an early NPC assembly intermediate, but not to mature NPCs. Heh2 recruits the endosomal sorting complex required for transport (ESCRT)-III subunit Snf7 and the AAA-ATPase Vps4 to destabilize and clear defective NPC assembly intermediates. When surveillance or clearance is compromised, malformed NPCs accumulate in a storage of improperly assembled nuclear pore complexes compartment, or SINC. The SINC is retained in old mothers to prevent loss of daughter lifespan, highlighting a continuum of mechanisms to ensure nuclear compartmentalization.

References

Nov 12, 2015·Journal of Molecular Biology·Nicole Monroe, Christopher P Hill
Nov 25, 2015·Critical Reviews in Biochemistry and Molecular Biology·April E RoseChristian Schlieker
Jan 18, 2016·Current Opinion in Cell Biology·Yolanda Olmos, Jeremy G Carlton
Dec 22, 2015·Cell·Christoph HagenKay Grünewald
Oct 7, 2015·Trends in Cell Biology·Brant M Webster, C Patrick Lusk
Oct 11, 2014·Cell·Greg Odorizzi
Mar 27, 2016·Current Opinion in Cell Biology·Marina VietriCoen Campsteijn
May 6, 2015·Nucleus·Brant M Webster, C Patrick Lusk
Apr 1, 2016·Current Opinion in Cell Biology·Coen CampsteijnHarald Stenmark
Apr 1, 2016·Current Opinion in Cell Biology·Megan C King, C Patrick Lusk
Jul 17, 2015·Molecular Biology of the Cell·Annemarie KraltLiesbeth M Veenhoff
Apr 12, 2016·Current Opinion in Cell Biology·Maria Makarova, Snezhana Oliferenko
May 27, 2015·ELife·Anna CaballeJuan Martin-Serrano
Jun 5, 2015·Nature·Yolanda OlmosJeremy G Carlton
Jun 5, 2015·Nature·Brian Burke
May 12, 2016·Developmental Cell·Rosemarie Ungricht, Ulrike Kutay
Sep 28, 2016·Trends in Biochemical Sciences·Liliane ChristHarald Stenmark
Jan 26, 2017·Nature Reviews. Molecular Cell Biology·Rosemarie Ungricht, Ulrike Kutay
Jan 7, 2017·Molecular Biology of the Cell·Natalie JohnsonGreg Odorizzi
Jul 21, 2017·Current Opinion in Cell Biology·Nicolas Chiaruttini, Aurélien Roux
Dec 1, 2017·Human Molecular Genetics·Samuel S PappasWilliam T Dauer
Nov 1, 2016·Molecular Biology of the Cell·Ethan LaudermilchChristian Schlieker
Feb 2, 2018·Molecular Biology of the Cell·Lauren PenfieldShirin Bahmanyar
Feb 14, 2018·The Journal of Cell Biology·Wanlu ZhangElmar Schiebel
Feb 26, 2016·Journal of Cell Science·Christophe LefebvreEmmanuel Culetto
Aug 11, 2018·Annual Review of Cell and Developmental Biology·John McCulloughWesley I Sundquist
Sep 5, 2015·F1000Research·Greg Odorizzi
Feb 6, 2019·Acta Neuropathologica·Katja Burk, R Jeroen Pasterkamp
Feb 17, 2018·Nature Communications·Toby MathiesonMikhail M Savitski
Dec 4, 2014·Nature Structural & Molecular Biology·John McCullough, Wesley I Sundquist
Apr 4, 2019·Annual Review of Biophysics·Bernhard HampoelzMartin Beck
Aug 28, 2015·The EMBO Journal·James H Hurley

Citations

Jul 21, 1995·The Journal of Biological Chemistry·E S JohnsonA Varshavsky
Dec 1, 1995·The Journal of Cell Biology·J D AitchisonMichael P Rout
May 16, 2001·The Journal of Cell Biology·M MarelliRichard W Wozniak
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Jul 23, 2003·Nature Biotechnology·Junmin PengSteven P Gygi
Oct 15, 2003·Proceedings of the National Academy of Sciences of the United States of America·Amy L HitchcockPamela A Silver
Aug 3, 2005·Nature Reviews. Molecular Cell Biology·Phyllis I Hanson, Sidney W Whiteheart
Sep 30, 2005·The EMBO Journal·Anna ScottWesley I Sundquist
Dec 2, 2006·Nature Reviews. Genetics·Brian C Capell, Francis S Collins
Jun 9, 2007·Science·Jez G Carlton, Juan Martin-Serrano
Oct 22, 2008·Journal of Molecular Biology·Malgorzata D GonciarzChristopher P Hill
Jan 13, 2009·Cell·Suraj SaksenaScott D Emr
Mar 11, 2009·The Journal of Cell Biology·T Renee DawsonSusan R Wente
May 6, 2009·The Journal of Cell Biology·Tadashi MakioRichard W Wozniak
Jun 16, 2009·Current Opinion in Cell Biology·Javier Fernandez-Martinez, Michael P Rout
Dec 19, 2009·Proceedings of the National Academy of Sciences of the United States of America·Kitty F VerzijlbergenFred van Leeuwen
Jan 23, 2010·Science·Michael CostanzoCharles Boone
Jan 30, 2010·Molecular Biology of the Cell·Anna ShestakovaMarkus Babst
May 26, 2010·The Journal of Cell Biology·Anne ChadrinValérie Doye
Jun 17, 2010·Cell·Christine M DoucetMartin W Hetzer
Jul 10, 2010·Proceedings of the National Academy of Sciences of the United States of America·Eiji MoritaWesley I Sundquist
Nov 26, 2010·The Journal of Cell Biology·Douglas R MackayKatharine S Ullman
Jan 26, 2011·The Journal of Cell Biology·Megan WemmerGreg Odorizzi
Feb 25, 2011·Molecular Biology of the Cell·William T YewdellC Patrick Lusk
Apr 23, 2011·Science·Nicholas RhindChad Nusbaum
Jul 6, 2011·The Journal of Cell Biology·Jessica A Talamas, Martin W Hetzer
Jan 4, 2012·The Journal of Cell Biology·Akira HayakawaCatherine Dargemont
Feb 4, 2012·Science·Jeffrey N SavasMartin W Hetzer
Mar 17, 2012·Science·Jeremy G CarltonJuan Martin-Serrano
May 9, 2012·Nucleus·Jesse D VargasMartin W Hetzer

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