Survey of the 1999 surface plasmon resonance biosensor literature
The application of surface plasmon resonance biosensors in life sciences and pharmaceutical research continues to increase. This review provides a comprehensive list of the commercial 1999 SPR biosensor literature and highlights emerging applications that are of general interest to users of the technology. Given the variability in the quality of published biosensor data, we present some general guidelines to help increase confidence in the results reported from biosensor analyses.
A mutational analysis of the binding of staphylococcal enterotoxins B and C3 to the T cell receptor beta chain and major histocompatibility complex class II
Homotypic interaction and multimerization of nucleocapsid protein of tomato spotted wilt tospovirus: identification and characterization of two interacting domains
On the importance of being aromatic at an antibody-protein antigen interface: mutagenesis of the extracellular interferon gamma receptor and recognition by the neutralizing antibody A6
BirA enzyme: production and application in the study of membrane receptor-ligand interactions by site-specific biotinylation
Probing the interaction between a high-affinity single-chain Fv and a pyrimidine (6-4) pyrimidone photodimer by site-directed mutagenesis
Differences in DNA recognition and conformational change activity between boxes A and B in HMG2 protein
Assembly of urokinase receptor-mediated plasminogen activation complexes involves direct, non-active-site interactions between urokinase and plasminogen
Structure-function analysis of the Z-DNA-binding domain Zalpha of dsRNA adenosine deaminase type I reveals similarity to the (alpha + beta) family of helix-turn-helix proteins
Biophysical characterization of the interaction of the beta-lactamase TEM-1 with its protein inhibitor BLIP
Role of phosphatidylinositol 3,4,5-trisphosphate in regulating the activity and localization of 3-phosphoinositide-dependent protein kinase-1
The optical biosensor studies on the role of hydrophobic tails of NADPH-cytochrome P450 reductase and cytochromes P450 2B4 and b5 upon productive complex formation within a monomeric reconstituted system
Folding, heterodimeric association and specific peptide recognition of a murine alphabeta T-cell receptor expressed in Escherichia coli
Solution structure of a retro-inverso peptide analogue mimicking the foot-and-mouth disease virus major antigenic site. Structural basis for its antigenic cross-reactivity with the parent peptide.
Synthetic peptides derived from the variable regions of an anti-CD4 monoclonal antibody bind to CD4 and inhibit HIV-1 promoter activation in virus-infected cells.
The salt dependence of DNA recognition by NF-kappaB p50: a detailed kinetic analysis of the effects on affinityand specificity
The effect of in vitro activation and platelet interaction on the CD9 distribution and adhesion properties of human eosinophils
Trading places on DNA--a three-point switch underlies primer handoff from primase to the replicative DNA polymerase
Single-chain variable fragments selected on the 57-76 p21Ras neutralising epitope from phage antibody libraries recognise the parental protein
Surface plasmon resonance measurements reveal stable complex formation between p53 and DNA polymerase alpha
The retinitis pigmentosa GTPase regulator, RPGR, interacts with the delta subunit of rod cyclic GMP phosphodiesterase
Immunogenicity and in vitro protective efficacy of a recombinant multistage Plasmodium falciparum candidate vaccine
The stabilized structural array of two HMG1/2-boxes endowed by a linker sequence between them is requisite for the effective binding of HMG1 with DNA
Different mechanisms are involved in the antibody mediated inhibition of ligand binding to the urokinase receptor: a study based on biosensor technology
Binding of the G domains of laminin alpha1 and alpha2 chains and perlecan to heparin, sulfatides, alpha-dystroglycan and several extracellular matrix proteins
Repertoire shift occurs during the memory maintenance phase of the immune responses and is not affinity-driven
PrKX is a novel catalytic subunit of the cAMP-dependent protein kinase regulated by the regulatory subunit type I.
The role of homophilic binding in anti-tumor antibody R24 recognition of molecular surfaces. Demonstration of an intermolecular beta-sheet interaction between vh domains.
Recombinant human peroxisomal targeting signal receptor PEX5. Structural basis for interaction of PEX5 with PEX14.
A soluble form of the avian hepatitis B virus receptor. Biochemical characterization and functional analysis of the receptor ligand complex.
Interaction of NE-dlg/SAP102, a neuronal and endocrine tissue-specific membrane-associated guanylate kinase protein, with calmodulin and PSD-95/SAP90. A possible regulatory role in molecular clustering at synaptic sites.
The L1 major capsid protein of human papillomavirus type 11 recombinant virus-like particles interacts with heparin and cell-surface glycosaminoglycans on human keratinocytes.
Binding and selective detection of the secretory N-terminal domain of the alzheimer amyloid precursor protein on cell surfaces
Importance of factor VIIa Gla-domain residue Arg-36 for recognition of the macromolecular substrate factor X Gla-domain
The inhibition of myeloperoxidase by ceruloplasmin can be reversed by anti-myeloperoxidase antibodies
Domain III of the Bacillus thuringiensis delta-endotoxin Cry1Ac is involved in binding to Manduca sexta brush border membranes and to its purified aminopeptidase N
DNA-Directed immobilization: efficient, reversible, and site-selective surface binding of proteins by means of covalent DNA-streptavidin conjugates
Poly(L-lysine)-graft-dextran copolymer promotes pyrimidine motif triplex DNA formation at physiological pH. Thermodynamic and kinetic studies.
Competition between thyroid hormone receptor-associated protein (TRAP) 220 and transcriptional intermediary factor (TIF) 2 for binding to nuclear receptors. Implications for the recruitment of TRAP and p160 coactivator complexes.
Demonstration of molecular interactions between the murein polymerase PBP1B, the lytic transglycosylase MltA, and the scaffolding protein MipA of Escherichia coli.
Studies on the interactions between human replication factor C and human proliferating cell nuclear antigen
A strategy for the generation of surfaces presenting ligands for studies of binding based on an active ester as a common reactive intermediate: a surface plasmon resonance study
Regulation of the catalytic function of coagulation factor VIIa by a conformational linkage of surface residue Glu 154 to the active site
Characterization of the interaction between the herpes simplex virus type I Fc receptor and immunoglobulin G.
Cluster of differentiation antigen 4 (CD4) endocytosis and adaptor complex binding require activation of the CD4 endocytosis signal by serine phosphorylation
T cell receptor and coreceptor CD8 alphaalpha bind peptide-MHC independently and with distinct kinetics
Qualitative and quantitative differences in T cell receptor binding of agonist and antagonist ligands
Sideroxylonal C, a new inhibitor of human plasminogen activator inhibitor type-1, from the flowers of Eucalyptus albens
Use of bacteriophage T7 displayed peptides for determination of monoclonal antibody specificity and biosensor analysis of the binding reaction
Sensitivity of real time viral detection by an optical biosensor system using a crude home-made antiserum against measles virus as a ligand
Structure-binding relationships for the interaction between a vancomycin monoclonal antibody Fab fragment and a library of vancomycin analogues and tracers
Fragment reconstitution of a small protein: disulfide mutant of a short C-terminal fragment derived from streptococcal protein G
Purified meningococcal transferrin-binding protein B interacts with a secondary, strain-specific, binding site in the N-terminal lobe of human transferrin
Kinetic analysis of the effect on Fab binding of identical substitutions in a peptide and its parent protein
Phosphatidyl choline-mediated inhibition of Streptococcus pneumoniae adherence to type II pneumocytes in vitro
The N-terminal CUB-epidermal growth factor module pair of human complement protease C1r binds Ca2+ with high affinity and mediates Ca2+-dependent interaction with C1s.
Kinetics of the interaction of endotoxin with polymyxin B and its analogs: a surface plasmon resonance analysis
Circular permutation of the granulocyte colony-stimulating factor receptor agonist domain of myelopoietin
Efficient site specific removal of a C-terminal FLAG fusion from a Fab' using copper(II) ion catalysed protein cleavage
Interaction of human macrophage C-type lectin with O-linked N-acetylgalactosamine residues on mucin glycopeptides.
Evidence of interactions between the nucleocapsid protein NCp7 and the reverse transcriptase of HIV-1.
Crystal structure of the CD2-binding domain of CD58 (lymphocyte function-associated antigen 3) at 1.8-A resolution
Optical biosensor studies on the productive complex formation between the components of cytochrome P450scc dependent monooxygenase system
Effects of microcystins on phosphorylase-a binding to phosphatase-2A: kinetic analysis by surface plasmon resonance biosensor
Deficiency in CD22, a B cell-specific inhibitory receptor, is sufficient to predispose to development of high affinity autoantibodies
Protein movement during complex-formation between tissue plasminogen activator and plasminogen activator inhibitor-1
Structural studies of the interactions of normal and abnormal human plasmins with bovine basic pancreatic trypsin inhibitor
Interaction of 14-3-3 with a nonphosphorylated protein ligand, exoenzyme S of Pseudomonas aeruginosa
Dimerization of profilin II upon binding the (GP5)3 peptide from VASP overcomes the inhibition of actin nucleation by profilin II and thymosin beta4
DNA binding properties of key sandramycin analogues: systematic examination of the intercalation chromophore
Interaction between Pleckstrin homology domains and G protein betagamma-subunits: analyses of kinetic parameters by a biosensor-based method
SPR analysis of the total reduction of protein adsorption to surfaces coated with mixtures of long- and short-chain polyethylene oxide block copolymers
Effect of methanol on the interaction of monoclonal antibody with free and immobilized atrazine studied using the resonant mirror-based biosensor
Vernier zone residue 4 of mouse subgroup II kappa light chains is a critical determinant for antigen recognition
An in vitro selected binding protein (affibody) shows conformation-dependent recognition of the respiratory syncytial virus (RSV) G protein
A phage-displayed mimotope inhibits tumour necrosis factor-alpha-induced cytotoxicity more effectively than the free mimotope
Epitope mapping of 10 monoclonal antibodies against the pig analogue of human membrane cofactor protein (MCP)
Shared and unique determinants of the erythropoietin (EPO) receptor are important for binding EPO and EPO mimetic peptide.
The kinetics of association and phosphorylation of IkappaB isoforms by IkappaB kinase 2 correlate with their cellular regulation in human endothelial cells
In vitro scanning saturation mutagenesis of all the specificity determining residues in an antibody binding site
Effects of humanization by variable domain resurfacing on the antiviral activity of a single-chain antibody against respiratory syncytial virus
Kinetic analysis of analyte binding by optical biosensors: hydrodynamic penetration of the analyte flow into the polymer matrix reduces the influence of mass transport
Structural and biochemical evaluation of the interaction of the phosphatidylinositol 3-kinase p85alpha Src homology 2 domains with phosphoinositides and inositol polyphosphates.
Inhibitory effect of a self-derived peptide on glucosyltransferase of Streptococcus mutans. Possible novel anticaries measures.
Detection of the deltaF508 (F508del) mutation of the cystic fibrosis gene by surface plasmon resonance and biosensor technology
Ligands for the tyrosine kinase p56lck SH2 domain: discovery of potent dipeptide derivatives with monocharged, nonhydrolyzable phosphate replacements
Syntheses of biotinylated alpha-galactosylceramides and their effects on the immune system and CD1 molecules
Endogenous proteins controlling amyloid beta-peptide polymerization. Possible implications for beta-amyloid formation in the central nervous system and in peripheral tissues.
The solution structure of FADD death domain. Structural basis of death domain interactions of Fas and FADD.
Spatiotemporal dynamics of inositol 1,4,5-trisphosphate that underlies complex Ca2+ mobilization patterns
Total alanine-scanning mutagenesis of insulin-like growth factor I (IGF-I) identifies differential binding epitopes for IGFBP-1 and IGFBP-3
The N-terminus of collagenase MMP-8 determines superactivity and inhibition: a relation of structure and function analyzed by biomolecular interaction analysis
Pseudouridine synthetase Pus1 of Saccharomyces cerevisiae: kinetic characterisation, tRNA structural requirement and real-time analysis of its complex with tRNA
Method for the preparation of bispecific F(ab')2mu fragments from mouse monoclonal antibodies of the immunoglobulin M class and characterization of the fragments
High-affinity binding of basic fibroblast growth factor and platelet-derived growth factor-AA to the core protein of the NG2 proteoglycan.
Divalent cations differentially regulate integrin alphaIIb cytoplasmic tail binding to beta3 and to calcium- and integrin-binding protein.
Major histocompatibility class I molecules present Urtica dioica agglutinin, a superantigen of vegetal origin, to T lymphocytes
Myosin light-chain kinase of smooth muscle stimulates myosin ATPase activity without phosphorylating myosin light chain
Phage-display library selection of high-affinity human single-chain antibodies to tumor-associated carbohydrate antigens sialyl Lewisx and Lewisx
Changes in the specificity of antibodies by site-specific mutagenesis followed by random mutagenesis
Binding characteristics and tumor targeting of a covalently linked divalent CC49 single-chain antibody
Real-time kinetics of HIV-1 Rev-Rev response element interactions. Definition of minimal binding sites on RNA and protein and stoichiometric analysis.
The transferrin receptor binding site on HFE, the class I MHC-related protein mutated in hereditary hemochromatosis
The intrinsic factor-vitamin B12 receptor, cubilin, is a high-affinity apolipoprotein A-I receptor facilitating endocytosis of high-density lipoprotein
A large non-immunized human Fab fragment phage library that permits rapid isolation and kinetic analysis of high affinity antibodies.
Characterization of vascular endothelial cell growth factor interactions with the kinase insert domain-containing receptor tyrosine kinase. A real time kinetic study.
Novel super-high affinity sheep monoclonal antibodies against CEA bind colon and lung adenocarcinoma
A cluster of positively charged amino acids in the C4BP alpha-chain is crucial for C4b binding and factor I cofactor function.
Specific interactions between Porphyromonas gingivalis fimbriae and human extracellular matrix proteins
Conformation of the primary binding loop folded through an intramolecular interaction contributes to the strong chymotrypsin inhibitory activity of the chymotrypsin inhibitor from Erythrina variegata seeds
Unique features of SHIP, SHP-1 and SHP-2 binding to FcgammaRIIb revealed by surface plasmon resonance analysis
Unique single-domain antigen binding fragments derived from naturally occurring camel heavy-chain antibodies
Interaction of BiP with the J-domain of the Sec63p component of the endoplasmic reticulum protein translocation complex.
Randomization of the receptor alpha chain recruitment epitope reveals a functional interleukin-5 with charge depletion in the CD loop.
Molecular dissection of the intrinsic factor-vitamin B12 receptor, cubilin, discloses regions important for membrane association and ligand binding.
Catfish Oct2 binding affinity and functional preference for octamer motifs, and interaction with OBF-1
Biochemical analysis of the interaction between elongation factor 1alpha and alpha/beta-tubulins from a ciliate, Tetrahymena pyriformis
Real time analysis of interaction between inositol 1,4, 5-trisphosphate receptor type I and its ligand
Identification and optimization of regeneration conditions for affinity-based biosensor assays. A multivariate cocktail approach
Vesicular-integral membrane protein, VIP36, recognizes high-mannose type glycans containing alpha1-->2 mannosyl residues in MDCK cells
Biosensor measurement of the interaction kinetics between insulin-like growth factors and their binding proteins
Expression and characterization of bispecific single-chain Fv fragments produced in transgenic plants
Tyrosine hydroxylase binds tetrahydrobiopterin cofactor with negative cooperativity, as shown by kinetic analyses and surface plasmon resonance detection
Crystal structure of the pleckstrin homology-phosphotyrosine binding (PH-PTB) targeting region of insulin receptor substrate 1
Potential drugs against cervical cancer: zinc-ejecting inhibitors of the human papillomavirus type 16 E6 oncoprotein
Conformational changes of gp120 in epitopes near the CCR5 binding site are induced by CD4 and a CD4 miniprotein mimetic
Application of peptide nucleic acid to the direct detection of deoxyribonucleic acid amplified by polymerase chain reaction
BIA/MS of epitope-tagged peptides directly from E. coli lysate: multiplex detection and protein identification at low-femtomole to subfemtomole levels
The N-terminus of gp130 is critical for the formation of the high-affinity interleukin-6 receptor complex
Analysis of receptor binding by the channel-forming toxin aerolysin using surface plasmon resonance.
Kinetics and peptide dependency of the binding of the inhibitory NK receptor CD94/NKG2-A and the activating receptor CD94/NKG2-C to HLA-E
Induction of vascular cell adhesion molecule-1 expression by IL-4 in human aortic smooth muscle cells is not associated with increased nuclear NF-kappaB levels
Novel phosphorylation sites of human tumour suppressor protein p53 at Ser20 and Thr18 that disrupt the binding of mdm2 (mouse double minute 2) protein are modified in human cancers
High-affinity interactions of tumor necrosis factor receptor-associated factors (TRAFs) and CD40 require TRAF trimerization and CD40 multimerization
Four A6-TCR/peptide/HLA-A2 structures that generate very different T cell signals are nearly identical
Characterization of a novel monoclonal antibody that senses nitric oxide-dependent activation of soluble guanylate cyclase
The light chain of factor VIII comprises a binding site for low density lipoprotein receptor-related protein.
Evidence by mutagenesis that Tyr(370) of the mouse ribonucleotide reductase R2 protein is the connecting link in the intersubunit radical transfer pathway.
Mapping the binding domains involved in the interaction between the Plasmodium falciparum knob-associated histidine-rich protein (KAHRP) and the cytoadherence ligand P. falciparum erythrocyte membrane protein 1 (PfEMP1).
Stromal cell-derived factor-1alpha associates with heparan sulfates through the first beta-strand of the chemokine.
Macromolecular substrate affinity for the tissue factor-factor VIIa complex is independent of scissile bond docking.
An analysis of the binding of repressor protein ModE to modABCD (molybdate transport) operator/promoter DNA of Escherichia coli.
Differential effects of single-stranded DNA binding proteins (SSBs) on uracil DNA glycosylases (UDGs) from Escherichia coli and mycobacteria
Both kinetic data and epitope mapping provide clues for understanding the anti-coagulant effect of five murine monoclonal antibodies to human beta2-glycoprotein I
The N- and C-terminal domains of MecA recognize different partners in the competence molecular switch
Construction of an artificial tandem protein of the c-Myb DNA-binding domain and analysis of its DNA binding specificity
Structural and functional characterization of an epitope in the conserved C-terminal region of HIV-1 gp120
Isolation and characterization of an anti-CD16 single-chain Fv fragment and construction of an anti-HER2/neu/anti-CD16 bispecific scFv that triggers CD16-dependent tumor cytolysis
The influence of signal noise on the accuracy of kinetic constants measured by surface plasmon resonance experiments
Glycation of apolipoprotein E impairs its binding to heparin: identification of the major glycation site
Mannose 6-Phosphate/Insulin-like growth factor II receptor mediates internalization and degradation of leukemia inhibitory factor but not signal transduction.
Trench-shaped binding sites promote multiple classes of interactions between collagen and the adherence receptors, alpha(1)beta(1) integrin and Staphylococcus aureus cna MSCRAMM.
Calmodulin inhibits inositol 1,4,5-trisphosphate-induced calcium release through the purified and reconstituted inositol 1,4,5-trisphosphate receptor type 1
Oligomeric complexes link Rab5 effectors with NSF and drive membrane fusion via interactions between EEA1 and syntaxin 13
alpha-synuclein binds to Tau and stimulates the protein kinase A-catalyzed tau phosphorylation of serine residues 262 and 356.
Mapping of the discontinuous H-kininogen binding site of plasma prekallikrein. Evidence for a critical role of apple domain-2.
p55-hGRF, a short natural form of the Ras-GDP exchange factor high yield production and characterization
The role of individual SH2 domains in mediating association of phospholipase C-gamma1 with the activated EGF receptor.
Investigations into self-association of vancomycin covalent dimers using surface plasmon resonance technology
Use of surface plasmon resonance for studies of protein-protein and protein-phospholipid membrane interactions. Application to the binding of factor VIII to von Willebrand factor and to phosphatidylserine-containing membranes
Biochemical characterization of the interaction between the Saccharomyces cerevisiae MSH2-MSH6 complex and mispaired bases in DNA.
Interaction of translation initiation factor eIF4G with eIF4A in the yeast Saccharomyces cerevisiae.
Isolation and characterization of bone morphogenetic protein-binding proteins from the early Xenopus embryo.
The kangaroo cation-independent mannose 6-phosphate receptor binds insulin-like growth factor II with low affinity.
Expression, purification, and functional analysis of murine ectodomain fragments of CD8alphaalpha and CD8alphabeta dimers.
Control of photoinduced electron transfer from zinc-porphyrin to methyl viologen by supramolecular formation between monoclonal antibody and zinc-porphyrin
Isolation of TBP-interacting protein (TIP) from a hyperthermophilic archaeon that inhibits the binding of TBP to TATA-DNA
Structural analysis of 14-3-3 phosphopeptide complexes identifies a dual role for the nuclear export signal of 14-3-3 in ligand binding
A novel immunological approach establishes that the auxin-binding protein, Nt-abp1, is an element involved in auxin signaling at the plasma membrane.
Killer cell immunoglobulin receptors and T cell receptors bind peptide-major histocompatibility complex class I with distinct thermodynamic and kinetic properties.
Hepatitis C virus core protein binds to apolipoprotein AII and its secretion is modulated by fibrates
Thermodynamics of T cell receptor binding to peptide-MHC: evidence for a general mechanism of molecular scanning
Effect of adjuvant composition on immune response to a multiple antigen peptide (MAP) containing a protective epitope from Neisseria meningitidis class 1 porin
Functionalization of covalent DNA-streptavidin conjugates by means of biotinylated modulator components
Screening the Biointeractions of Submicron Sized Particles Intended for Site-Specific Delivery Using Surface Plasmon Resonance
Characterization of the 2:1 complex between the class I MHC-related Fc receptor and its Fc ligand in solution
Biosynthesis and immunobiochemical characterization of gp17/GCDFP-15. A glycoprotein from seminal vesicles and from breast tumors, in HeLa cells and in Pichia pastoris yeast
Identification of a neuritogenic ligand of the neural cell adhesion molecule using a combinatorial library of synthetic peptides
Surface plasmon resonance screening of synthetic peptides mimicking the immunodominant region of C-S8c1 foot-and-mouth disease virus
Effects of mutations in the F361 to R364 region of topoisomerase I (Topo I), in the presence and absence of 9-aminocamptothecin, on the Topo I-DNA interaction
Core-directed protein design. I. An experimental method for selecting stable proteins from combinatorial libraries
An NMR analysis of ubiquitin recognition by yeast ubiquitin hydrolase: evidence for novel substrate recognition by a cysteine protease
Formation and biochemical characterization of tube/pelle death domain complexes: critical regulators of postreceptor signaling by the Drosophila toll receptor
Surface plasmon resonance studies resolve the enigmatic endotoxin neutralizing activity of polymyxin B.
Differential binding of histidine-rich glycoprotein (HRG) to human IgG subclasses and IgG molecules containing kappa and lambda light chains.
Direct interaction of the trans-Golgi network membrane protein, TGN38, with the F-actin binding protein, neurabin.
The linker region between the helicase and primase domains of the bacteriophage T7 gene 4 protein is critical for hexamer formation.
The HU protein from Thermotoga maritima: recombinant expression, purification and physicochemical characterization of an extremely hyperthermophilic DNA-binding protein
Biochemical analysis of SopE from Salmonella typhimurium, a highly efficient guanosine nucleotide exchange factor for RhoGTPases.
Structural features required for the interaction of the Hsp70 molecular chaperone DnaK with its cochaperone DnaJ.
Identification of Tek/Tie2 binding partners. Binding to a multifunctional docking site mediates cell survival and migration.
Interaction between collagens and glycosaminoglycans investigated using a surface plasmon resonance biosensor
Binding and antigen presentation of ceramide-containing glycolipids by soluble mouse and human CD1d molecules
Metal ion selectivity for formation of the calmodulin-metal-target peptide ternary complex studied by surface plasmon resonance spectroscopy
Kinetics of apolipoprotein E isoforms-binding to the major glycosaminoglycans of the extracellular matrix
Interaction of pulmonary surfactant protein A with phospholipid liposomes: a kinetic study on head group and fatty acid specificity
Development and validation of an immunoreceptor assay for simulect based on surface plasmon resonance
Characterization of the carbohydrate binding specificity and kinetic parameters of lectins by using surface plasmon resonance
Real-time study of interactions between a composite DNA regulatory region (HIV-1 LTR NRE) and several transcription factors of nuclear extracts
Multiple domains of DFF45 bind synergistically to DFF40: roles of caspase cleavage and sequestration of activator domain of DFF40
Mutations in the rod outer segment membrane guanylate cyclase in a cone-rod dystrophy cause defects in calcium signaling
The second and fourth cluster of class A cysteine-rich repeats of the low density lipoprotein receptor-related protein share ligand-binding properties.
Reduced toxicity of expression, in Escherichia coli, of antipollutant antibody fragments and their use as sensitive diagnostic molecules
Biosynthesis of vascular endothelial growth factor-D involves proteolytic processing which generates non-covalent homodimers.
A novel NE-dlg/SAP102-associated protein, p51-nedasin, related to the amidohydrolase superfamily, interferes with the association between NE-dlg/SAP102 and N-methyl-D-aspartate receptor.
The variable region-1 from tissue-type plasminogen activator confers specificity for plasminogen activator inhibitor-1 to thrombin by facilitating catalysis: release of a kinetic block by a heterologous protein surface loop
The interaction of DNA gyrase with the bacterial toxin CcdB: evidence for the existence of two gyrase-CcdB complexes
The hRad51 and RecA proteins show significant differences in cooperative binding to single-stranded DNA
Specific interaction of the RNA-binding domain of the bacillus subtilis transcriptional antiterminator GlcT with its RNA target, RAT
Binding analysis of Xenopus laevis translation initiation factor 4E (eIF4E) in initiation complex formation
Triplex formation of chemically modified homopyrimidine oligonucleotides: thermodynamic and kinetic studies
Cyclic AMP-dependent protein kinase binding to A-kinase anchoring proteins in living cells by fluorescence resonance energy transfer of green fluorescent protein fusion proteins.
Interaction of the human NF-kappaB p52 transcription factor with DNA-PNA hybrids mimicking the NF-kappaB binding sites of the human immunodeficiency virus type 1 promoter.
An engineered minidomain containing an elastin turn exhibits a reversible temperature-induced IgG binding
Structural basis of glycosaminoglycan modification and of heterotypic interactions of perlecan domain V
Tryptophan H33 plays an important role in pyrimidine (6-4) pyrimidone photoproduct binding by a high-affinity antibody
Affinity analysis of non-steady-state data obtained under mass transport limited conditions using BIAcore technology
The influence of transport on the kinetics of binding to surface receptors: application to cells and BIAcore
Antibody affinities and relative titers in polyclonal populations: surface plasmon resonance analysis of anti-DNA antibodies
Analysis of human serum antibody-carbohydrate interaction using biosensor based on surface plasmon resonance
Surface plasmon resonance biosensor studies of human wild-type and mutant lecithin cholesterol acyltransferase interactions with lipoproteins
Initiation of Escherichia coli ribosomes on matrix coupled mRNAs studied by optical biosensor technique
Resistance of the human immunodeficiency virus to the inhibitory action of negatively charged albumins on virus binding to CD4
Antigen-antibody interactions: binding studies with fluorescence and surface plasmon resonance exemplified by acid-traseolide as antigen
Activation of human neutrophils by a synthetic anti-microbial peptide, KLKLLLLLKLK-NH2, via cell surface calreticulin
Enovin, a member of the glial cell-line-derived neurotrophic factor (GDNF) family with growth promoting activity on neuronal cells. Existence and tissue-specific expression of different splice variants
Both G-type domains of protein S are required for the high-affinity interaction with C4b-binding protein
Identification and characterization of potential new therapeutic targets in inflammatory and autoimmune diseases
Histidine-rich glycoprotein prevents the formation of insoluble immune complexes by rheumatoid factor
Synergistic transcriptional activation by hGABP and select members of the activation transcription factor/cAMP response element-binding protein family.
Mapping and characterization of the epitope(s) of Sch 55700, a humanized mAb, that inhibits human IL-5
Lipid-induced conformation and lipid-binding properties of cytolytic and antimicrobial peptides: determination and biological specificity
Kinetic characterization of the interaction of the Z-fragment of protein A with mouse-IgG3 in a volume in chemical space
The inhibitory receptor LIR-1 uses a common binding interaction to recognize class I MHC molecules and the viral homolog UL18
The leucine-based sorting motifs in the cytoplasmic domain of the invariant chain are recognized by the clathrin adaptors AP1 and AP2 and their medium chains
Binding of 14-3-3 protein to the plasma membrane H(+)-ATPase AHA2 involves the three C-terminal residues Tyr(946)-Thr-Val and requires phosphorylation of Thr(947)
Modulation of actomyosin ATPase by thiotetromycin is mediated through conformational change of actin
Characterization of monoclonal antibodies to proteinase 3 (PR3) as candidate tools for epitope mapping of human anti-PR3 autoantibodies
Evidence that 3-phosphoinositide-dependent protein kinase-1 mediates phosphorylation of p70 S6 kinase in vivo at Thr-412 as well as Thr-252
Selection of phage-displayed fab antibodies on the active conformation of ras yields a high affinity conformation-specific antibody preventing the binding of c-Raf kinase to Ras
Mapping part of the functional epitope for ligand binding on the receptor for urokinase-type plasminogen activator by site-directed mutagenesis
RNA recognition by transcriptional antiterminators of the BglG/SacY family: functional and structural comparison of the CAT domain from SacY and LicT
Functional properties of complement factor H-related proteins FHR-3 and FHR-4: binding to the C3d region of C3b and differential regulation by heparin
Binding of Bacillus thuringiensis Cry1Ac toxin to Manduca sexta aminopeptidase-N receptor is not directly related to toxicity
Low affinity binding of an LFA-3/IgG1 fusion protein to CD2+ T cells is independent of cell activation
Summary report of the TD-3 workshop: characterization of 83 antibodies against prostate-specific antigen
Surface plasmon resonance (SPR) analysis of coagulation in whole blood with application in prothrombin time assay
Selection of phage-displayed anti-guinea pig C5 or C5a antibodies and their application in xenotransplantation
Analysis of A-kinase anchoring protein (AKAP) interaction with protein kinase A (PKA) regulatory subunits: PKA isoform specificity in AKAP binding
A paradigm shift is needed in proteomics: 'structure determines function' should be replaced by 'binding determines function'
Fluorescence resonance energy transfer and complex formation between thiazole orange and various dye-DNA conjugates: implications in signaling nucleic acid hybridization
Determination of affinities and antigenic epitopes of bovine cardiac troponin I (cTnI) with monoclonal antibodies by surface plasmon resonance biosensor
Detection and characterization of pediatric serum antibody to the OMP P5-homologous adhesin of nontypeable Haemophilus influenzae during acute otitis media
Optical biosensor analysis in studying herpes simplex virus glycoprotein D binding to target nectin1 receptor
Studying the assembly of multicomponent protein and ribonucleoprotein complexes using surface plasmon resonance
Surface plasmon resonance: towards an understanding of the mechanisms of biological molecular recognition
Minimal structural elements of an inhibitory anti-ATF1/CREB single-chain antibody fragment (scFv41.4)
Direct comparison of binding equilibrium, thermodynamic, and rate constants determined by surface- and solution-based biophysical methods
Coping with complexity (in macromolecular interactions)--a comment on Rebecca L. Rich's and David G. Myszka's "grading the commercial optical biosensor literature--class of 2008: 'the mighty binders"'
Comparative analysis of 10 small molecules binding to carbonic anhydrase II by different investigators using Biacore technology
Biosensor-surface plasmon resonance: quantitative analysis of small molecule-nucleic acid interactions
Exploiting Surface Plasmon Resonance (SPR) Technology for the Identification of Fibroblast Growth Factor-2 (FGF2) Antagonists Endowed with Antiangiogenic Activity
Using complementary acoustic and optical techniques for quantitative monitoring of biomolecular adsorption at interfaces
SPR studies of carbohydrate-protein interactions: signal enhancement of low-molecular-mass analytes by organoplatinum(II)-labeling
Quantitative investigation of the direct interaction between Hemagglutinin and fusion proteins of Peste des petits ruminant virus using surface Plasmon resonance
Surface plasmon resonance imaging for affinity analysis of aptamer-protein interactions with PDMS microfluidic chips
Kinetic analysis of the interaction between amphotericin B and human serum albumin using surface plasmon resonance and fluorescence spectroscopy
Mining the oncoproteome and studying molecular interactions for biomarker development by 2DE, ChIP and SPR technologies
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Myocardial stunning is a mechanical dysfunction that persists after reperfusion of previously ischemic tissue in the absence of irreversible damage including myocardial necrosis. Here is the latest research.
Pontocerebellar hypoplasias are a group of neurodegenerative autosomal recessive disorders with prenatal onset, atrophy or hypoplasia of the cerebellum, hypoplasia of the ventral pons, microcephaly, variable neocortical atrophy and severe mental and motor impairments. Here is the latest research on pontocerebellar hypoplasia.
Cell Atlas Along the Gut-Brain Axis
Profiling cells along the gut-brain axis at the single cell level will provide unique information for each cell type, a three-dimensional map of how cell types work together to form tissues, and insights into how changes in the map underlie health and disease of the GI system and its crosstalk with the brain. Disocver the latest research on single cell analysis of the gut-brain axis here.
Chronic Traumatic Encephalopathy
Chronic Traumatic Encephalopathy (CTE) is a progressive degenerative disease that occurs in individuals that suffer repetitive brain trauma. Discover the latest research on traumatic encephalopathy here.