Survey of Virulence Determinants among Vancomycin Resistant Enterococcus faecalis and Enterococcus faecium Isolated from Clinical Specimens of Hospitalized Patients of North west of Iran.

The Open Microbiology Journal
Yaeghob SharifiMorteza Milani

Abstract

Recent data indicates an increasing rate of vancomycin resistance in clinical enterococcal isolates worldwide. The nosocomial enterococci are likely to harbor virulence elements that increase their ability to colonize hospitalized patients. The aim of this study was to characterize virulence determinants in vancomycin-resistant enterococci (VRE) obtained from various clinical sources.During the years 2008 to 2010, a total of 48 VRE isolates were obtained from three University teaching hospitals in Northwest, Iran. Initially, phenotypic speciation was done and minimum inhibitory concentrations (MICs) of vancomycin were determined by agar dilution method and E-test. Then, species identification and resistance genotypes along with detection of virulence genes (asa1, esp, gelE, ace and cpd) of the isolates were performed by multiplex PCR.Thirty eight isolates were identified as vancomycin-resistant Enterococcus faecium (VREfm) and ten as E. faecalis (VREfs). Irrespective of the species, vanA gene (89.58%) was dominant and three phenotypically vancomycin susceptible E. faecium isolates carried the vanB gene. Among virulence genes investigated, the esp was found in 27(71%) VREfm strains, but did not in any VREfs. Other virulence dete...Continue Reading

Citations

Feb 7, 2015·Indian Journal of Medical Microbiology·Y A HashemM A Amin
Aug 2, 2013·Memórias do Instituto Oswaldo Cruz·Carolina Baldisserotto ComerlatoPedro Alves d'Azevedo
May 20, 2017·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Ali JahansepasJalal Mardaneh
Jan 22, 2017·Environmental Science and Pollution Research International·Dalel BelhajHabib Ayadi
Feb 12, 2017·Chonnam Medical Journal·Mohammad Reza ArabestaniSeyed Masoud Mousavi
May 1, 2018·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Ali JahansepasMohammad Aghazadeh
Mar 28, 2019·FEMS Microbiology Letters·Maniya ArshadiMohammad Reza Pourmand
Feb 6, 2021·Pathogens·Gustavo Henrique Rodrigues Vale de MacedoLuís Cláudio Nascimento da Silva

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