PMID: 30148319Aug 1, 2016Paper

Survey on Toxoplasma gondii Infection in Depressed Patients and Genotype Identification in Guizhou Province

Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases
Cai-yan ZouYong-nian Li

Abstract

To survey on Toxoplasma gondii infection in depressed patients in Guizhou Province and identify the genotype of T. gondii. Enzyme-linked immunosorbent assay (ELISA) was applied to detect the T. gondii-specific antibodies IgG, IgM and circulating antigens (CAg) of T. gondii in 141 patients and 150 healthy subjects. The specific repeated DNA fragment (529 bp) of T. gondii was amplified by PCR. The genotype of T. gondii was determined by multiplex multilocus nested polymerase chain reaction-restriction fragment length polymorphism (Mn-PCR-RFLP). ELISA showed that the positive rate of anti-T. gondii antibody in depressed patients and healthy subjects was 21.3%(30/141) and 7.3%(11/150), respectively. The positive rate of IgG in depressed patients was 18.4% (26/141), significantly higher than that in healthy subjects (7.3%, 11/150)(P<0.05). The positive rate of IgM and CAg in depressed patients was both 1.4% (2/141), while these were not found in healthy subjects. PCR revealed one patient positive for T. gondii, whose genotype was further identified to be Toxo DB #9(Chinese 1 type) by Mn-PCR-RFLP. The positive rate of T. gondii is higher in depressed patients than in the healthy subjects in Guizhou Province. The genotype of T. gondi...Continue Reading

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