Survey protocol for detecting chytridiomycosis in all Australian frog populations

Diseases of Aquatic Organisms
Lee F SkerrattRichard Speare

Abstract

Spread of the amphibian chytrid fungus Batrachochytrium dendrobatidis (Bd) has caused the decline and extinction of frogs, but the distribution of Bd is not completely known. This information is crucial to implementing appropriate quarantine strategies, preparing for outbreaks of chytridiomycosis due to introduction of Bd, and for directing conservation actions towards affected species. This survey protocol provides a simple and standard method for sampling all frog populations in Australia to maximise the chances of detecting Bd. In order to structure and prioritise the protocol, areas are divided by bioregion and frog species are allocated depending on the water bodies they utilize into 3 groups representing different levels of risk of exposure to Bd. Sixty individuals per population need to be tested to achieve 95% certainty of detecting 1 positive frog, based on the minimum apparent prevalence of > or =5% in infected Australian frog populations and using a quantitative real-time TaqMan PCR test. The appropriate season to sample varies among bioregions and will ideally incorporate temperatures favourable for chytridiomycosis (e.g. maximum air temperatures generally <27 degrees C). Opportunistic collection and testing of sick...Continue Reading

Citations

Mar 17, 2012·Diseases of Aquatic Organisms·Oliver J Hyman, James P Collins
Sep 13, 2012·Diseases of Aquatic Organisms·Kim F Hauselberger, Ross A Alford
Jan 16, 2015·Diseases of Aquatic Organisms·Sanjay MolurNeelesh Dahanukar
Jul 1, 2014·Conservation Biology : the Journal of the Society for Conservation Biology·Ben C ScheeleDon A Driscoll
Mar 20, 2014·Conservation Biology : the Journal of the Society for Conservation Biology·Annemarieke Spitzen-Van Der SluijsFrank Pasmans
Mar 31, 2010·Ecological Applications : a Publication of the Ecological Society of America·Michael J AdamsMatthias Leu
Jun 13, 2015·Proceedings. Biological Sciences·Elizabeth A RoznikRoss A Alford
Mar 10, 2015·Diseases of Aquatic Organisms·Andrée-Michelle D'Aoust-MessierDavid Lesbarrères
Apr 23, 2015·PloS One·Elodie A CourtoisDirk S Schmeller
May 14, 2016·PloS One·Sofía Rodríguez-BrenesMichael J Ryan
Nov 26, 2013·Diseases of Aquatic Organisms·Torsten OhstJörg Plötner
Apr 29, 2020·Ecological Applications : a Publication of the Ecological Society of America·Sara C BellLee F Skerratt
Apr 30, 2018·EFSA Journal·UNKNOWN EFSA Panel on Animal Health and Welfare (AHAW)Christian Gortázar Schmidt
Jun 8, 2021·Journal of the Indian Institute of Science·Gayathri Sreedharan, Karthikeyan Vasudevan
Aug 3, 2021·Frontiers in Veterinary Science·Deanna H OlsonAndrew R Blaustein

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