Survival and activity of Pseudomonas sp. strain B13(FR1) in a marine microcosm determined by quantitative PCR and an rRNA-targeting probe and its effect on the indigenous bacterioplankton.

Applied and Environmental Microbiology
T D LeserN B Hendriksen

Abstract

Genetically engineered Pseudomonas sp. strain B13(FR1) was released into laboratory-scale marine ecosystem models (microcosms). Survival of the introduced population in the water column and the sediment was determined by plating on a selective medium and by quantitative competitive PCR. The activity of the released bacteria was determined by in situ hybridization of single cells with a specific rRNA-targeting oligonucleotide probe. Two microcosms were inoculated with 10(6) cells ml-1, while an uninoculated microcosm served as a control. The number of Pseudomonas sp. strain B13(FR1) cells decreased rapidly to ca. 10(2) cells ml-1 within 2 days after the release, which is indicative of grazing by protozoa. Three days after the introduction into seawater, cells were unculturable, but PCR continued to detect cells in low numbers. Immediately after the release, the ribosomal content of Pseudomonas sp. strain B13(FR1) corresponded to a generation time of 2 h. The growth rate decreased to less than 0.04 h-1 in 5 days and remained low, probably because of carbon limitation of the cells. Specific amendment of the microcosms with 10 mM 4-chlorobenzoate resulted in a rapid increase of the growth rate and an exponentially increasing number...Continue Reading

References

May 1, 1977·Applied and Environmental Microbiology·J E HobbieS Jasper
Feb 1, 1992·Applied and Environmental Microbiology·R I AmannD A Stahl
Sep 1, 1992·Applied and Environmental Microbiology·C PicardP Simonet
Apr 1, 1992·Applied and Environmental Microbiology·R PipkeD F Dwyer
Feb 1, 1992·Applied and Environmental Microbiology·Y L Tsai, B H Olson
Sep 1, 1991·Applied and Environmental Microbiology·L A BraunsJ D Oliver
Sep 1, 1987·Microbiological Reviews·D B Roszak, R R Colwell
May 1, 1988·Applied and Environmental Microbiology·D A StahlL Montgomery
Sep 1, 1982·Applied and Environmental Microbiology·L N LiangM Alexander
Jan 1, 1981·Annual Review of Microbiology·M Alexander
Apr 1, 1994·Applied and Environmental Microbiology·S M ThiemJ M Tiedje
Jul 1, 1993·Nucleic Acids Research·N LarsenC R Woese
Jul 1, 1956·Journal of General Microbiology·D HERBERTR C TELLING
Jun 1, 1988·Applied and Environmental Microbiology·J A FuhrmanA M Chan
Aug 1, 1993·Applied and Environmental Microbiology·P F KempJ Laroche
Sep 1, 1993·Microbial Ecology·B C Sander, J Kalff
Dec 1, 1990·Microbial Ecology·K R Gurijala, M Alexander

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Citations

Jul 23, 2003·Environmental Microbiology·M Jahangir AlamSumio Shinoda
Apr 6, 2002·FEMS Microbiology Letters·Muhammad Jahangir AlamSumio Shinoda
Dec 7, 2005·FEMS Microbiology Ecology·Charles W KnappDavid W Graham
May 19, 2000·FEMS Microbiology Ecology·C J PhillipsJ I Prosser
Oct 6, 1998·Applied and Environmental Microbiology·C A Kreader
Jun 3, 1998·Applied and Environmental Microbiology·B B ChristensenS Molin
Mar 21, 1998·Applied and Environmental Microbiology·K Reilly, G T Attwood
Oct 20, 2005·Journal of Bioscience and Bioengineering·K Watanabe, P W Baker
May 2, 2000·Applied and Environmental Microbiology·D B OertherL Raskin
Jul 9, 2004·Applied and Environmental Microbiology·Freddie H SharkeyRoger Marchant
Sep 4, 2004·Applied and Environmental Microbiology·Mark A RandaEelin Lim
Nov 1, 1996·Applied and Environmental Microbiology·J P Gray, R P Herwig
Nov 27, 2002·Applied and Environmental Microbiology·Robert A KanalyKazuya Watanabe
Jul 1, 1997·Applied and Environmental Microbiology·V G MathesonL J Forney
Oct 1, 1995·Applied and Environmental Microbiology·C PitulleG E Fox
Feb 1, 1996·Applied and Environmental Microbiology·M T Suzuki, S J Giovannoni

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