Survival and functions of encapsulated porcine hepatocytes after allotransplantation or xenotransplantation without immunosuppression

Surgery
S BenoistB Nordlinger

Abstract

This study evaluated the survival and functions of encapsulated porcine hepatocytes after intraperitoneal allotransplantation and xenotransplantation without immunosuppression. Isolated porcine hepatocytes were encapsulated in AN 69 polymer capsules (45.10(6)/capsule) and transplanted intraperitoneally in 12 rats and 12 pigs. Fifteen, 30, and 60 days after transplantation, capsules were removed and the viability and morphology of explanted hepatocytes were examined under light and electronic microscopy. The potential to produce albumin was assessed by evaluating the level of albumin messenger RNA, using semiquantitative reverse transcription-polymerase chain reaction. 6beta-Hydroxylase activity was measured by high-performance liquid chromatography. In addition, cytochrome P450 3A proteins were detected by Western blot only in allogeneic hepatocytes. Similar results were observed after allotransplantation and xenotransplantation. Histologic studies showed that hepatocytes were well-preserved and arranged in cords for up to 30 days. The expression of porcine albumin gene was maintained up to 15 days. 6beta-Hydroxylase activity was 2.5-fold lower at day 15 than in freshly encapsulated hepatocytes, which were not transplanted. In ...Continue Reading

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Citations

Jul 25, 2009·Tissue Engineering. Part C, Methods·Delphine Logeart-AvramoglouHerve Petite
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Jan 8, 2015·Expert Opinion on Drug Delivery·Haritz GurruchagaJose Luis Pedraz
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May 9, 2012·The International Journal of Artificial Organs·Liyuan QiuChangyong Wang

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