PMID: 3748047Sep 1, 1986Paper

Survival and mutagenesis of bacteriophage T7 damaged by methyl methanesulfonate and ethyl methanesulfonate

Mutation Research
L A Dodson, W E Masker

Abstract

We have examined survival and mutagenesis of bacteriophage T7 after exposure to the alkylating agents methyl methanesulfonate (MMS) and ethyl methanesulfonate (EMS). It was found that although both alkylating agents caused increased reversion of specific T7 mutations, EMS caused a higher frequency of reversion than did MMS. Exposure of the host cells to ultraviolet light so as to induce the SOS system resulted in increased survival (Weigle reactivation) of T7 phage damaged with either EMS or MMS. However, after SOS induction of the host we did not detect an accompanying increase in mutation frequency measured as either reversion of specific T7 mutants or by generation of mutations in the T7 gene that codes for phage ligase. Neither mutation frequency nor survival of alkylated phage was affected by the umuD,C mutation in the Escherichia coli host nor by the presence of plasmid pKM101. This may mean that the mode of Weigle reactivation that is detected in T7 is not mutagenic in nature.

References

Jan 1, 1979·Cold Spring Harbor Symposia on Quantitative Biology·M RadmanS Spadari
Dec 15, 1977·Journal of Molecular Biology·C Coulondre, J H Miller
Apr 1, 1985·Journal of Bacteriology·L Marsh, G C Walker
Nov 1, 1969·Virology·F W Studier
Oct 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·E L LoechlerJ M Essigmann
Mar 8, 1984·Nature·J S EadieM D Topal
Oct 1, 1983·Journal of Bacteriology·L A Dodson, W E Masker
Feb 26, 1981·Nature·C J Kenyon, G C Walker
Dec 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·L A DodsonW E Masker
Jul 1, 1980·Journal of Bacteriology·N K Notani, J K Setlow
Jul 1, 1953·Proceedings of the National Academy of Sciences of the United States of America·J J Weigle

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Citations

Jan 1, 1987·Mutation Research·L M Scearce, W E Masker

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