Survival and recovery of Escherichia coli O157:H7, Salmonella, and Listeria monocytogenes on lettuce and parsley as affected by method of inoculation, time between inoculation and analysis, and treatment with chlorinated water

Journal of Food Protection
Megan M LangLarry R Beuchat

Abstract

The effects of method for applying inoculum and of drying time after inoculation on survival and recovery of foodborne pathogens on iceberg lettuce and parsley were studied. Five-strain mixtures of Escherichia coli O157:H7, Salmonella, or Listeria monocytogenes were applied to lettuce and parsley by dip, spot, or spray inoculation methods. Inocula were dried for 2 h at 22 degrees C or for 2 h at 22 degrees C and then 22 h at 4 degrees C before being treated with water (control) or chlorine (200 microg/ml). Significantly higher populations (CFU per lettuce or parsley sample) of E. coli O157:H7 and Salmonella (alpha = 0.05) were recovered from dip-inoculated produce than from spot- or spray-inoculated produce. This difference was attributed to larger numbers of cells adhering to lettuce and parsley subjected to dip inoculation. Populations of E. coli O157:H7 and Salmonella recovered from lettuce inoculated by spot and spray methods were not significantly different, but populations recovered from spot-inoculated parsley were significantly higher than those recovered from spray-inoculated parsley, even though the number of cells applied was the same. Significantly different numbers of L. monocytogenes were recovered from inoculated...Continue Reading

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Citations

Oct 24, 2007·Journal of Applied Microbiology·N DreuxC Nguyen-The
Sep 8, 2010·Journal of Food Protection·Wenjing Pan, Donald W Schaffner
Jun 11, 2019·International Journal of Food Science·Ahmad Ni'matullah Al-BaarriShigeru Hayakawa
Jan 14, 2020·Journal of Food Protection·Sophia C PintonLaura Kathryn Strawn
Feb 23, 2020·Frontiers in Plant Science·Cristián Jacob, Maeli Melotto
Aug 18, 2009·Journal of Food Protection·Guodong ZhangMichael P Doyle
Aug 18, 2009·Journal of Food Protection·Helga J DoeringWilliam L Kerr
Apr 30, 2017·Food Science and Biotechnology·Jin-Ha HwangKi-Hwan Park
Sep 7, 2007·Journal of Food Protection·Pascal DelaquisLaura-Dorina Dinu
May 5, 2006·Applied and Environmental Microbiology·Andrew J HamiltonGlenn Hale
Oct 14, 2008·Foodborne Pathogens and Disease·Irene B HanningSteven C Ricke
Aug 12, 2009·Applied and Environmental Microbiology·Siyun WangWei Zhang
Jun 1, 2011·Journal of Food Science·Latha MurugesanAnuradha Prakash
Sep 15, 2011·PloS One·Adam SchikoraHeribert Hirt
Apr 12, 2016·Journal of Environmental Quality·Michael A JahneSeungo Kim
Dec 18, 2016·Applied and Environmental Microbiology·Cameron ParsonsSophia Kathariou
Jul 20, 2010·Environmental Microbiology·Cedric N BergerGad Frankel
Jul 5, 2012·Journal of Food Science·Gülten Tiryaki GündüzMehmet Karapinar
Apr 12, 2018·Foodborne Pathogens and Disease·Jitendra PatelJennifer Green
Oct 4, 2011·Applied and Environmental Microbiology·Laura-Dorina Dinu, Susan Bach

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