Survival features of EBV-stabilized cells from centenarians: morpho-functional and transcriptomic analyses.

Paola MatarreseW Malorni


In the present work, we analyzed the survival features of six different Epstein-Barr virus (EBV)-stabilized lymphoid cell lines obtained from adult subjects and from subjects of more than 95 years. For the first, we found that lymphoid B cells from centenarians were more resistant to apoptosis induction and displayed a more developed lysosomal compartment, the most critical component of phagic machinery, in comparison with lymphoid B cells from adult subjects. In addition, cells from centenarians were capable of engulfing and digesting other cells, i.e., their siblings (even entire cells), whereas lymphoid cells from "control samples", i.e., from adults, did not. This behavior was improved by nutrient deprivation but, strikingly, it was unaffected by the autophagy-modulating drug, rapamycin, an autophagy inducer, and 3-methyladenine, an autophagy inhibitor. Transcriptomic analyses indicated that: (1) aspartyl proteases, (2) cell surface molecules such as integrins and cadherins, and (3) some components of cytoskeletal network could contribute to establish this survival phenotype. Also, Kyoto Encyclopedia of Genes and Genomes pathways such as Wnt signaling pathway, an essential contributor to cell migration and actin cytoskeleto...Continue Reading


May 10, 2000·Nature Medicine·G Kroemer, J C Reed
Jun 10, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Paola MatarreseW Malorni
Jun 23, 2004·Trends in Immunology·Andras PerlKatalin Banki
Jan 20, 2005·Molecular and Cellular Biology·Patricia BoyaG Kroemer
Oct 26, 2005·Cell Death and Differentiation·Patrice Codogno, A J Meijer
Mar 7, 2006·Mechanisms of Ageing and Development·S AlbertiDaniela Monti
Dec 19, 2006·Autophagy·W MalorniM Piacentini
Sep 18, 2007·Experimental Gerontology·P SansoniM Passeri
Nov 17, 2007·Cell Host & Microbe·Christian Münz
Dec 7, 2007·Autophagy·Anthony Orvedahl, Beth Levine
Mar 13, 2008·Médecine sciences : M/S·Arnaud A MailleuxJoan Brugge
May 28, 2008·Cellular Microbiology·Anthony Orvedahl, Beth Levine
Sep 12, 2008·Nature Reviews. Molecular Cell Biology·Michael Overholtzer, Joan Brugge
Dec 3, 2008·Neuroimmunomodulation·R OstanClaudio Franceschi
Feb 5, 2009·Bioinformatics·João Pedro de MagalhãesGeorge M Church
Apr 15, 2009·Cell Research·Shih-Lei LaiRandall T Moon
Apr 21, 2009·Autophagy·Derek NarendraRichard J Youle
Aug 27, 2009·Cellular and Molecular Life Sciences : CMLS·K PankováJan Brábek
Oct 3, 2009·The Journal of Cell Biology·Anne Simonsen, Sharon A Tooze
Oct 20, 2009·Nature Cell Biology·Jong-Soo LeeJae U Jung
Dec 4, 2009·Cell Research·Youcun Qian, Yufang Shi
Jan 5, 2010·Current Opinion in Cell Biology·Kevin MoreauD C Rubinsztein
Jan 22, 2010·American Journal of Physiology. Cell Physiology·Maryam MehrpourPatrice Codogno
Feb 13, 2010·Current Opinion in Immunology·Christian Münz
Jul 20, 2010·Biochimica Et Biophysica Acta·Suning ChenJian Zhang
Jul 24, 2010·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Yiyang LaiBoon-Huat Bay
Sep 3, 2010·Nature Cell Biology·Zhifen Yang, Daniel J Klionsky
Sep 3, 2010·Nature Cell Biology·Frank MadeoG Kroemer


Jun 6, 2013·Future Medicinal Chemistry·Francesca Aredia, A Ivana Scovassi
Dec 6, 2014·BMC Complementary and Alternative Medicine·Chenjun HaoWeihui Zhang

Datasets Mentioned


Methods Mentioned

flow cytometry
transmission electron microscopy
nucleotide exchange

Related Concepts

Programmed Cell Death, Type II
Cell Line, Transformed
Cell Motility
Cell Survival
Epstein-Barr Virus
Lymphoid Cells
Liver Cell Adhesion Molecule

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Adherens Junctions

An adherens junction is defined as a cell junction whose cytoplasmic face is linked to the actin cytoskeleton. They can appear as bands encircling the cell (zonula adherens) or as spots of attachment to the extracellular matrix (adhesion plaques). Adherens junctions uniquely disassemble in uterine epithelial cells to allow the blastocyst to penetrate between epithelial cells. Discover the latest research on adherens junctions here.

Autophagy & Model Organisms

Autophagy is a cellular process that allows degradation by the lysosome of cytoplasmic components such as proteins or organelles. Here is the latest research on autophagy & model organisms

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.

Related Papers

Current Biology : CB
Oliver FloreyMichael Overholtzer
The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology
Yue-Qin Yang, Ji-Cheng Li
Biochimica Et Biophysica Acta
Jean-Claude Martinou, G Kroemer
© 2021 Meta ULC. All rights reserved