Survival Mechanisms of Campylobacter hepaticus Identified by Genomic Analysis and Comparative Transcriptomic Analysis of in vivo and in vitro Derived Bacteria

Frontiers in Microbiology
Thi Thu Hao VanRobert J Moore

Abstract

Chickens infected with Campylobacter jejuni or Campylobacter coli are largely asymptomatic, however, infection with the closely related species, Campylobacter hepaticus, can result in Spotty Liver Disease (SLD). C. hepaticus has been detected in the liver, bile, small intestine and caecum of SLD affected chickens. The survival and colonization mechanisms that C. hepaticus uses to colonize chickens remain unknown. In this study, we compared the genome sequences of 14 newly sequenced Australian isolates of C. hepaticus, isolates from outbreaks in the United Kingdom, and reference strains of C. jejuni and C. coli, with the aim of identifying virulence genes associated with SLD. We also carried out global comparative transcriptomic analysis between C. hepaticus recovered from the bile of SLD infected chickens and C. hepaticus grown in vitro. This revealed how the bacteria adapt to proliferate in the challenging host environment in which they are found. Additionally, biochemical experiments confirmed some in silico metabolic predictions. We found that, unlike other Campylobacter sp., C. hepaticus encodes glucose and polyhydroxybutyrate metabolism pathways. This study demonstrated the metabolic plasticity of C. hepaticus, which may c...Continue Reading

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Citations

Apr 26, 2020·Applied and Environmental Microbiology·Samiullah KhanKapil K Chousalkar
Aug 13, 2020·New Zealand Veterinary Journal·T R CrawshawA C Midwinter
Mar 27, 2021·Microbiology Resource Announcements·Ananta ArukhaSubhashinie Kariyawasam
Mar 31, 2021·Critical Reviews in Food Science and Nutrition·Karolinny Cristiny de Oliveira VieiraLizziane Kretli Winkelstroter Eller

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Datasets Mentioned

BETA
CP031611.1
PRJNA485661
CP031611

Methods Mentioned

BETA
PCR
RNA Assay
Illumina
RNA-Seq
glycosylation

Software Mentioned

ABRicate
CRISPRFinder
SEED viewer
Prokka
Bacterial Pan genomes analysis ( BPGA
Interproscan
BPGA
RAxML
RAMI
DNAplotter

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Campylobacteriosis (ASM)

Campylobacteriosis is caused by the bacteria Campylobacter jejuni and is a common cause of gastroenteritis in humans. Discover the latest research on Campylobacteriosis here.